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CFPAC1 cells fixed and labeled with 5 μg/mL of GCTM-5 antibody (green); cells are also labeled with DAPI (blue) to visualize all nuclei. A subset of t...
Western Blot Analysis: CFPAC1 total cell lysates were resolved by electrophoresis, transferred to PVDF and probed with the GCTM-5 antibody (2.5 µg/m...
Anti-GCTM-5, clone GCTM-5
| 种属反应性 | 关键应用 | 宿主 | 形式 | 抗体类型 |
|---|---|---|---|---|
| H | IC, IH, WB | Mouse | Purified | Monoclonal Antibody |
Description:
GCTM-5 Antibody, clone GCTM-5
Clone:
GCTM-5
Trade Name:
Chemicon (Millipore)
Background Information:
The GCTM-5 monoclonal antibody reacts with a minority sub-population of cells in spontaneously differentiating cultures of pluripotent human embryonic stem cells and embryonal carcinoma. The epitope recognized by GCTM-5 is found on a 50-kDa protein present on the surface of these cells. Previous studies suggest that GCTM-5 may also prove to be a useful tool for defining cell lineage relationships between putative progenitor populations in embryonic liver and biliary epithelium during tissue repair (Pera M, et al, 2005).
Key Applications:
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
Species Reactivity:
Human
Species Reactivity Note:
Reacts with human. Reactivity to other species has not yet been determined.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Molecular Weight:
~ 50 kDa
Stem Cell Type:
- Human Embryonic Stem Cells
- Pancreatic Stem Cells
Stem Cell Workflow Stage:
Characterization
Immunogen:
Membrane prepared from testicular seminoma
Format:
Purified
Presentation:
Purified mouse monoclonal IgG1 with 0.05% sodium azide.
Storage Conditions:
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
Host:
Mouse
Isotype:
IgG1
Antibody Category:
Stem Cell Research
Antibody Sub-Category:
Pluripotent & Early Differentiation
Antibody Market:
Stem Cells
Concentration:
1 mg/mL
Antibody Type:
Monoclonal Antibody
Specificity:
This antibody reacts with GCTM-5 antigen that is expressed upon the cell surface of human embryonal carcinoma, differentiating cultures of human embryonic stem cells, and bilary epithelial cells.