Información pedidos
Rat Leptin RIA
Description:
Rat Leptin RIA
Background Information:
Leptin is synthesized in adipocytes as a 16kD molecule and the circulating level is directly proportional to the total amount of fat in the body.
Key Applications:
Radioimmunoassay
Application Notes:
Two-day, disequilibrium incubation at room tempature. Sample volume 100 µL serum, plasma, or tissue culture media.
Species Reactivity:
Rat
Kit or Assay Type:
- Endocrine Kits
- Radioimmunoassays (RIA)
Therapeutic Areas:
- Obesity
- Metabolic Disorders
Linearity of Dilution:
78–96%
Label:
125I-Rat Leptin
Precision, %:
- Inter-assay: 3.0–5.7
- Intra-assay: 2.0–4.6
Accuracy:
92–103%
Detection Methods:
125I
Performance:
- ED80 = 1.0 ± 0.1 nh/ml
- ED50 = 3.1 ± 0.2 ng/ml
- ED20 = 9.9 ± 1.0 ng/ml
Standard Curve Range:
0.5–50 ng/mL
Specificity:
Rat Leptin 100% Mouse Leptin 100% Insulin Not Detectable Human Leptin <2% Glucagon Not Detectable SRIF Not Detectable Canine Leptin 0.5%
Analytes Available:
Leptin