Ordering Information
Product Images
The extent of carbonylation was calculated for each test concentration of hydrogen peroxide and the results are depicted in this graph. As would be e...
Flow Chart of OxyELISA™ Protein Quantitation Procedure.
OxyELISA™ Oxidized Protein Quantitation Kit
Description:
OxyELISA™ Oxidized Protein Quantitation Kit
Trade Name:
OxyELISA
Product Overview:
The OxyELISA™ Oxidized Protein Quantitation Kit provides a sensitive methodology for detection and quantitation of proteins modified by oxygen free radicals and other reactive species. Oxygen-derived free radicals have been implicated in numerous physiological functions such as apoptosis, cancer, aging, neurodegenerative diseases, chronic inflammatory diseases, pulmonary diseases, and cardiovascular diseases (for reviews, see ref. 1-4).
Oxygen free radicals are generated by environmental factors such as ionizing radiation and chemical substances. They are also produced during normal cellular metabolism by mitochondrial electron transport, the cellular redox system, and by immune responses (1, 5-7). Although cells have developed various antioxidant defenses to protect against free radicals (7-9), free radicals that escape the defenses attack and modify subcellular components – nucleic acids, lipids and proteins (1, 10-12). In some cases, cells respond to the oxidative stimuli and allow the organism to adapt to the oxidative stress (13-15).
Proteins are one of the major targets of oxygen free radicals and other reactive species. Oxidation of proteins modifies the side chains of methionine, histidine, and tyrosine and forms cysteine disulfide bonds (16-19). Metal catalyzed oxidation of proteins introduces carbonyl groups (aldehydes and ketones) at lysine, arginine, proline or threonine residues in a site-specific manner (1, 16, 20-22).
The oxidative modification of proteins can modulate biochemical characteristics of proteins such as enzymatic activity (21-23), DNA binding activities of transcription factors (24-26), and the susceptibility to proteolytic degradation (12, 25-28). While a relationship between protein oxidation and aging has been suggested (29-31), little is known about the importance of oxidative modification of individual proteins in the pathophysiology of free radical mediated processes.
In conclusion, the OxyELISA Oxidized Protein Quantitation Kit provides chemical and immunological reagents necessary to perform quantitative detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.
For Research Use Only; Not for use in diagnostic procedures
Oxygen free radicals are generated by environmental factors such as ionizing radiation and chemical substances. They are also produced during normal cellular metabolism by mitochondrial electron transport, the cellular redox system, and by immune responses (1, 5-7). Although cells have developed various antioxidant defenses to protect against free radicals (7-9), free radicals that escape the defenses attack and modify subcellular components – nucleic acids, lipids and proteins (1, 10-12). In some cases, cells respond to the oxidative stimuli and allow the organism to adapt to the oxidative stress (13-15).
Proteins are one of the major targets of oxygen free radicals and other reactive species. Oxidation of proteins modifies the side chains of methionine, histidine, and tyrosine and forms cysteine disulfide bonds (16-19). Metal catalyzed oxidation of proteins introduces carbonyl groups (aldehydes and ketones) at lysine, arginine, proline or threonine residues in a site-specific manner (1, 16, 20-22).
The oxidative modification of proteins can modulate biochemical characteristics of proteins such as enzymatic activity (21-23), DNA binding activities of transcription factors (24-26), and the susceptibility to proteolytic degradation (12, 25-28). While a relationship between protein oxidation and aging has been suggested (29-31), little is known about the importance of oxidative modification of individual proteins in the pathophysiology of free radical mediated processes.
In conclusion, the OxyELISA Oxidized Protein Quantitation Kit provides chemical and immunological reagents necessary to perform quantitative detection of carbonyl groups introduced into proteins by oxidative reactions with ozone or oxides of nitrogen or by metal catalyzed oxidation.
For Research Use Only; Not for use in diagnostic procedures
Key Applications:
ELISA
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
- Oxidative Stress Kits
- Apoptosis Assays
- Neurobiology Kits
- ELISA Assays
Therapeutic Areas:
- Cancer
- Neurodegenerative Disease
- Aging
Components:
2,4-Dinitrophenylhydrazine (DNPH) Solution 1.1mL
Oxidized Protein Standard (Lyophilized) 1 vial
Reduced Protein Standard (Lyophilized) 1 vial
5x Lysis Buffer/Protein Diluent 20mL
10x Blocking Buffer 6mL
10x Wash Buffer 130mL
Anti-DNP Mouse Monoclonal HRP Conjugated Antibody 22μL
3,3’,5,5’-Tetramethylbenzidine (TMB) Solution 45mL
Stop Solution 12mL
MILLEX® GP Syringe Driven Filter Unit (0.22μm)(Catalog No. SLGP033RS) 3 Filters
Adhesive Plate Sealing Films 6 Films
High Protein Binding Assay Plate 2 Plates (96-well)
Oxidized Protein Standard (Lyophilized) 1 vial
Reduced Protein Standard (Lyophilized) 1 vial
5x Lysis Buffer/Protein Diluent 20mL
10x Blocking Buffer 6mL
10x Wash Buffer 130mL
Anti-DNP Mouse Monoclonal HRP Conjugated Antibody 22μL
3,3’,5,5’-Tetramethylbenzidine (TMB) Solution 45mL
Stop Solution 12mL
MILLEX® GP Syringe Driven Filter Unit (0.22μm)(Catalog No. SLGP033RS) 3 Filters
Adhesive Plate Sealing Films 6 Films
High Protein Binding Assay Plate 2 Plates (96-well)
Storage Conditions:
Maintain at 2-8°C for up to 1 year after date of receipt.
Detection Methods:
Colorimetric