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Sensitive Rat Insulin RIA


Description:
Sensitive Rat Insulin RIA
Background Information:
Insulin, a 5.8 kDa hormone is secreted by the islet beta cells in the pancreas. This kit offers sensitive assay for rat/mouse insulin
Key Applications:
Radioimmunoassay
Application Notes:
Two-day disequilibrium incubation at 4°C. Sample volume 100 μL serum, plasma, or tissue culture media.
Species Reactivity:
Rat
Kit or Assay Type:
  • Endocrine Kits
  • Radioimmunoassays (RIA)
Therapeutic Areas:
  • Obesity
  • Metabolic Disorders
Linearity of Dilution:
84–119%
Label:
125I-Insulin
Precision, %:
  • Inter-assay: 3.8–10.8
  • Intra-assay: 2.7–5.8
Accuracy:
88–108%
Detection Methods:
125I
Performance:
  • ED80 = 0.09 ± 0.01 ng/mL
  • ED50 = 0.22 ± 0.01 ng/mL
  • ED20 = 0.55 ± 0.01 ng/mL
Standard Curve Range:
0.02–1.0 ng/mL
Specificity:
Rat Insulin I 100% Rat Insulin II 100% Human Insulin 100% Porcine Insulin 100% Sheep Insulin 100% Hamster Insulin 100% Mouse Insulin 100% Rat C-Peptide Not Detectable Glucagon Not Detectable Somatostatin Not Detectable Pancreatic Polypeptide No