Montage® Antibody Purification Kits

A new spin on antibody purification

Montage Antibody Purification Kits are designed for fast and easy antibody purification from serum, ascites or cell culture supernatants. The kits include spin columns pre-packed with high capacity PROSEP-A or G media. There are no pumps to setup. No columns to pack. All you need is a centrifuge to purify 10–20 mg in less than 60 minutes.

The protocol is easy to follow and, unlike traditional gravity flow methods, the use of centrifugal purification allows for walk-away convenience.

Results show that the spin columns can be regenerated up to 10X with no loss of binding capacity or reduced
specificity.

Performance (return to top)

Spin Column Reuse

Human IgG was purified 10 times from normal serum using a regenerated PROSEP-G spin column.

Purification of MAbs

Monoclonal mouse IgG was purified with a PROSEP-A spin column from ascites. (Lane 1: unpurified ascites. Lane 2: flow-through ascites. Lane 3: wash flow-through. Lane 4: 5 µg purified antibody.) Courtesy Exalpha Biologicals.

Binding Affinities of Protein A and Protein G
Protein	A	G		Protein	A	G
Human IgG1	++	++		Rat IgM	+ /--	-
Human IgG2	++	++		Rabbit IgG	++	++
Human IgG3	--	++		Hamster IgG	+	++
Human IgG4	++	++		Guinea Pig IgG	++	+
Human IgA	+	--		Bovine IgG	+	+
Human IgD	+	--		Sheep IgG	+ / --	+
Human IgE	+	--		Goat IgG	+ /--	+
Human IgM	+	--		Pig IgG	++	++
Mouse IgG1	+	+		Chicken IgG	--	+ /--
Mouse IgG2a	++	++		Fragments
Mouse IgG2b	++	++		Human Fab	+	+
Mouse IgG3	+	++		Human F(ab')2	+	+ /--
Mouse IgM	+ /--	--		Human ScFv	+	--
Rat IgG	++	++		Human Fc	+	--
Rat IgG1	+ /--	+		Human K	--	--
Rat IgG2a	+ /--	++		Human	--	+
Rat IgG2b	+ /--	+
Rat IgG2c	+ /--	+
Legend: ++ = Strong affinity; + = Moderate/slight affinity; +/-- = Requires evaluation; -- = No affinity

Specifications (return to top)

Kit Contents	Includes spin columns, buffers, sample preparation and concentration devices
Supporting Matrix Material	Porous glass
Media Particle Size, µm	75–125
Media Bed Volume, µL	1600
Binding Capacity
Protein A	>20 mg of rabbit IgG
Protein G	>10 mg of rabbit IgG
pH Range	2–9
Maximum Volume, mL	20 (maximum in swinging bucket rotor)
Storage Temperature, °C	2–8 for media. Buffer at room temperature
Color Coded End-caps
Protein A	Red
Protein G	Yellow

Ordering Information  (return to top)

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