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Microcon® Centrifugal Filter Units

The lab standard for sub-microliter volumes

       • Solute recoveries typically >95%, ideal for dilute (ng/mL to µg/mL range) protein solutions
       • Patented deadstop for reproducible concentrate volumes
       • Inverted spin method of concentrate retrieval maximizes recovery
       • Low-binding Ultracel membrane
       • Color coding indicates MW cut-off
       • Convenient sample storage of filtrate or concentrated sample in standard microfuge collection tube
       • Use in standard 1.5 mL tube fixed-angle rotors

Applications

       • Concentrating and purifying proteins, antibodies and nucleic acids (alternative to EtOH precipitation)
       • Desalting and buffer exchange
       • Removal of primers, linkers and unincorporated label

Typical Protein Recovery

Retentate Recovery (%)
Solute (Concentration)Nominal MW3103050100
Bovine IgG Fraction II (1 mg/mL)156,0009595959595
Bovine Serum Albumin (1 mg/mL)67,0009595959050
Ovalbumin (1 mg/mL)45,0009595959010
α-Chymotrypsinogen (1 mg/mL)25,00095959580
Cytochrome c (0.25 mg/mL)12,40095959010

Specifications (return to top)

Volumes
Maximum Initial Sample Volume, mL0.5
Typical Final Concentrate Volume, µL5–15
Centrifugal Force*Fixed-angle microfuge rotor w/maximum 14,000 x g
Materials
MembraneUltracel regenerated cellulose
DeviceSample reservoir: polypropylene; Sample vial: polycarbonate
Membrane Support BaseAcetal
O-ringMedical grade silicone rubber
Dimensions
Active Membrane Area, cm20.32
Diameter, mm12.3
Length, in concentration mode, mm45
*500 x g for DNA/RNA applications with Microcon YM-100 Filter Unit

New Amicon Ultra-0.5 mL filters

Available in 3,000 and 10,000 MWCO, the vertical membrane design of Amicon Ultra-0.5 mL filters allows equal to or better sample concentration than Microcon filters, but in half the spin times.


Ordering Information  (return to top)

Please select 5 or fewer Items to compare.

Compare Catalogue No. Product Name NMWL, kDa Color Code Qty/Pk  
42422 Microcon YM-30 Centrifugal Filter Unit 30 Clear 8 » Add to Cart
42409 Microcon YM-30 Centrifugal Filter Unit 30 Clear 24 » Add to Cart
42410 Microcon YM-30 Centrifugal Filter Unit 30 Clear 100 » Add to Cart
42411 Microcon YM-30 Centrifugal Filter Unit 30 Clear 500 » Add to Cart
42423 Microcon YM-50 Centrifugal Filter Unit 50 Rose 8 » Add to Cart
42415 Microcon YM-50 Centrifugal Filter Unit 50 Rose 24 » Add to Cart
42416 Microcon YM-50 Centrifugal Filter Unit 50 Rose 100 » Add to Cart
42424 Microcon YM-100 Centrifugal Filter Unit 100 Blue 8 » Add to Cart
42412 Microcon YM-100 Centrifugal Filter Unit 100 Blue 24 » Add to Cart
42413 Microcon YM-100 Centrifugal Filter Unit 100 Blue 100 » Add to Cart
42414 Microcon YM-100 Centrifugal Filter Unit 100 Blue 500 » Add to Cart
2 receiver tubes are included with each filter unit. Additional receiver tubes can be ordered separately (100/pk; cat. no. 1065601)