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Montage Plasmid Miniprep96 Kit
Montage Plasmid Miniprep96 Kit 是一种快速、易于使用的可制备少量高纯度质粒试剂盒。 由于采用了拥有专利的分离技术，这种 Montage 试剂盒只需一个简便的实验方案，而无需冗长的吸附/洗脱和离心步骤，只需不到传统方法 50% 的时间即可获得高纯度的质粒DNA，而且具有很好的重现性。试剂盒包括 96 孔质粒 DNA 纯化所需的所有试剂和材料。 细菌裂解之后，只需三步简单的过滤，即可从每块板制备 96 个纯化 DNA 样品。 纯化样品适用于最灵敏的下游应用，包括克隆、DNA 测序、转化及其它应用。Montage Plasmid Miniprep96 Kit 适用于自动化液体操作系统。 可从板上收集纯化样品且无需进行离心处理，扩大了试剂盒适用的自动化系统范围。
For BAC DNA protocol, see Montage Plasmid MiniprepHTS Kit user guide, P36315
高质量
使用 Montage Plasmid Miniprep96 Kit（分子量标记 - λ Hind III 酶切物）纯化的质粒 DNA 的琼脂糖凝胶图谱质量和重复性都很好。
BAC DNA purified with the Montage Plasmid MiniprepHTS 96 Kit was dissolved in 35 µL of the kit's Resuspension Buffer (solution 5) and 10 µL of the DNA were used for sequencing in a BigDye version 3.0 sequencing reaction (3/8 scale) as described in the kit's optimized protocol. The sequencing reaction was cleaned up using Montage SEQ384 plate and injected onto the ABI 3700 capillary sequencer.
Chromosomal DNA
DNA from three different BAC clones from a human chromosome 22 human BAC DNA library (Research Genetics, Inc.) was purified with the Montage Plasmid MiniprepHTS 96 Kit and electrophoresed on a 0.8% agarose gel for 120 min at 100 V either uncut (lanes 1 – 3) or after digestion with Not I (lanes 4 – 6) or Hind III (lanes 7 – 9). Lane M is a Hind III digest of Lambda phage DNA. The BAC DNA purified with the Montage Plasmid MiniprepHTS 96 Kit is suitable for fingerprinting.
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