Amicon Ultra-15 Centrifugal Filter Units

The premier device for concentrating <15 mL

• ● High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
• ● High retentate recovery of >90%
• ● Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
• ● Heat-sealed membrane minimizes downstream extractables
• ● 100% integrity tested for reliable performance
• ● Convenient sample monitoring with translucent housing and volume gradations
• ● Direct pipettor sample access eliminates processing step to recover concentrate
• ● High concentration factors of 80–100X

Applications

• ● Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
• ● Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
• ● Concentration of dilute or pre-purified proteins from column eluents
• ● Desalting, buffer exchange and protein dialysis
• ● Size exclusion and protein fractionation

Typical Spin Times to Filtrate Volume

Spin conditions: 4000 xg swinging bucket rotor at 25°C, 15 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery (return to top)

Specifications (return to top)

Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane is CE marked.