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Amicon® Ultra-4 Centrifugal Filter Units

Provide both ultra-fast spin times and ultra-high recoveries
 

  • High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
  • High retentate recovery of >90%
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)
  • Heat-sealed membrane minimizes downstream extractables
  • 100% integrity tested for reliable performance
  • Convenient sample monitoring with translucent housing and volume gradations
  • Direct pipette sample access eliminates processing step to recover concentrate
  • High concentration factors of 80–100X

Applications

  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC
  • Concentration of dilute or pre-purified proteins from column eluents
  • Desalting, buffer exchange and protein dialysis
  • Size exclusion and protein fractionation

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Typical Spin Times to Filtrate Volume

Spin 
conditions: 4000 xg swinging bucket rotor at 25 °C, 4 mL sample. <br 
/>3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 
50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Spin conditions: 4000 xg swinging bucket rotor at 25 ° C, 4 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery

Membrane
NMWL, kDa
Protein Solute Protein Retentate
Volume, mL
Protein Retentate
Recovery, %
3 Cytochrome c, 12.4 kDa
(0.25 mg/mL)
0.19 98.7
10 Cytochrome c, 12.4 kDa
(0.25 mg/mL)
0.20 95
30 BSA, 67 kDa
(1 mg/mL)
0.20 95
50 BSA, 67 kDa
(1 mg/mL)
0.08 94
100 IgG, 156 kDa
(1 mg/mL)
0.34 91
4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).

Amicon Ultra filter units process 4 mL samples as fast as 5 minutes


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