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ZipTip® Pipette Tips

For concentrating and purifying femtomoles to picomoles of biomolecules

    ZipTip is a 10 µL pipette tip with a 0.6 or 0.2 µL bed of chromatography media fixed at its end with no dead volume. It is ideal for concentrating and purifying peptides or proteins in seconds prior to mass spectrometry, HPLC, capillary electrophoresis and other analytical
    techniques.

    The ZipTip pipette tip is simple and easy to use. Place the tip on a single- or multi-channel pipettor, standard 22-gauge blunt-end HPLC needle, or compatible automated liquid handling/sample preparation station. For sample binding, aspirate and dispense through the media several times. Contaminates are then washed away in similar fashion. The concentrated, purified sample is eluted in 1–4 µL of compatible solvent with direct transfer to a mass spectrometer target or vial. For applications requiring smaller elution volumes (e.g., <1 µL), a micro-bed format containing 0.2 µL of media is available.

    The ZipTip pipette tip provides a reproducible, high recovery method for concentrating and purifying femtomoles to picomoles of peptides, proteins and oligonucleotides for improved data quality. For simplified analysis, fractionation of complex peptide mixtures by step elution is also possible.

       • Ready to use
       • Eliminates labor-intensive
    chromatography techniques

Resins for a Variety of Applications

Performance

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Specifications (return to top)

Materials
Pipette TipPolypropylene
C18 and µ-C18 ResinSilica, 15 µm, 200 Å pore size
C4 ResinSilica, 15 µm, 300 Å pore size
SCX ResinStrong cation exchange, 12 µm, 300 Å pore size
Volumes, µL
Pipette tip10
Resin Bed0.2 or 0.6
Capacity
ZipTipC18Typically 5 µg when used with saturating amounts of analyte
ZipTipµ-C18Typically 2.0 µg when used with saturating amounts of analyte
ZipTipC4Typically 3.3 µg when used with saturating amounts of analyte
Dimensions
Tip Length, mm31
Tip ID (top), mm3.375
Tip OD (top), mm5.8
Temperature, °C4–70
pH Range1.5–13.5; 2–12 for 24 hour exposure

Recommended Equipment

Manual Liquid Handling:
ZipTip pipette tips are compatible with most 10 µL single-channel pipettors. The Biohit Proline™ electronic multi-channel pipettor is recommended for simultaneously processing eight samples.
Automated Liquid Handling:
ZipTip pipette tips are also proven for use on a variety of automated liquid handling systems including:

       • ABI SymBiot® Sample Workstations for Biospectrometry™ with universal cone for ZipTip tips (ABI catalogue no. GEN602987) and rack adapter for ZipTip box
       • Genomic Solutions ProMS™ Sample Preparation Station
       • Bruker Daltonics MAP™ II and II/8 MALDI AutoPrep Systems with rack adapter for ZipTip box (Millipore catalogue no. ST0B DAP TR)
       • Tecan GENESIS Workstation
       • CyBio CyBi™-Well 96 Automated Pipettor


Informações para Pedidos  (retornar ao início)

Por favor selecione 05 (cinco) ou menos itens para comparar.

Comparar Número de catálogo Descrição Qtde/cx  
ZTC18S008 Ponteira de Pipeta ZipTipC18 com resina C18 8 » Adicionar ao pedido
ZTC18S096 Ponteira de Pipeta ZipTipC18 com resina C18 96 » Adicionar ao pedido
ZTC18S960 Ponteira de Pipeta ZipTipC18 com resina C18 960 » Adicionar ao pedido
ZTC04S008 Ponteira de Pipeta ZipTipC4 com resina C4 8 » Adicionar ao pedido
ZTC04S096 Ponteira de Pipeta ZipTipC4 com resina C4 96 » Adicionar ao pedido
ZTC04S960 Ponteira de Pipeta ZipTipC4 com resina C4 960 » Adicionar ao pedido
ZTC18M008 Ponteira de Pipeta ZipTipµ-C18 com micro-leito de resina C18 8 » Adicionar ao pedido
ZTC18M096 Ponteira de Pipeta ZipTipµ-C18 com micro-leito de resina C18 96 » Adicionar ao pedido
ZTC18M960 Ponteira de Pipeta ZipTipµ-C18 com micro-leito de resina C18 960 » Adicionar ao pedido
ZT0MCS008 Ponteira de Pipeta ZipTipMC com resina para quelação de metais 8 » Adicionar ao pedido
ZT0MCS096 Ponteira de Pipeta ZipTipMC com resina para quelação de metais 96 » Adicionar ao pedido
ZTSCXS008 ZipTip with 0.6 µL strong cation resin 8 » Adicionar ao pedido
ZTSCXS024 ZipTip with 0.6 µL strong cation resin 24 » Adicionar ao pedido
ZTSCXS096 ZipTip with 0.6 µL strong cation resin 96 » Adicionar ao pedido