Concentration and purification of biological solutions
High Throughput UF Technology
MultiScreen filter plate with Ultracel-10 membrane provides a new method for high throughput sample prep. The
ultrafiltration-based filter plate is designed for automation-compatible sample purification, concentration and desalting of biological solutions, as well as protein removal from samples prior to instrument analysis.
The 96-well MultiScreen filter plate incorporates Ultracel-10 10,000 kDa nominal molecular weight limit regenerated cellulose ultrafiltration membrane for low-binding, high recovery results. It is designed for use with centrifugation and is compatible with standard microtiter plates, instrumentation, and liquid handling equipment.
Reliable
MultiScreen filter plate with Ultracel-10 membrane is designed for low protein binding and high protein retention. The assay-grade plates meet stringent manufacturing release specifications and are lot tested for protein retention for >95% retention of cytochrome c (12,500 Daltons). They also offer high well-to-well uniformity on performance.
Versatile Operation
MultiScreen filter plate with Ultracel-10 membrane allows for processing and collection of sample volumes from 50 to 500 µL. They are compatible with a range of standard receiver 96-well microtiter plates including 300 µL, 700 µL deep well and 150 µL conical well.
MultiScreen filter plate with Ultracel-10 membrane is ideal for applications including protein concentration and desalting prior to validation or screening assays. They are able to remove large molecular weight proteins prior to instrument analysis.
Performance
![MB5093-71[1159-EN].jpg](/images/medium/MB5093-71[1159-EN].jpg)
Different protein solutes with an initial sample volume of 300 µL were spun at 2000 x g. Ultrafiltrate volumes are lower for samples with higher protein concentrations such as serum.
Typical Protein Recovery
| Membrane NMWL, kDa | Protein Solute | Typical Retention |
| 10,000 | Cytochrome c
12,500 kDa (1 mg/mL) | 95% |
| 10,000 | BSA
67,000 kDa (1 mg/mL) | 99% |
| Volumes |
| Filter Plate Capacity, mL | 0.5 |
| Working Sample Volume Capacity | Limited to receiver plate. Compatible with standard 96-well microtiter plates (300 µL, 700 µL deep well, and 150 µL conical u-bottom).* |
| Centrifugal Force | Maximum 3,000 x g |
| Materials |
| Membrane | Ultracel regenerated cellulose |
| Device | Polyolefin |
| Dimensions |
| Length, mm | 128 |
| Width, mm | 85.2 |
| Height, mm | 14.2 |
Required Equipment (not included)
• Centrifuge capable of a minimum of 2000 x g with a swinging bucket rotor and 96-well plate carrier
• Pipetters or robotic liquid handlers for transferring 10 µL, 25 µL and 300 µL volumes
• 96-well microtiter plate receiver tray
Recommended Receiver Plates
• Millipore Solvinert Collection Plate for UV analysis: cat. no. MSCP NPP 00
• Millipore Deep Well Collection Block: cat. no. MDCP N2M 50
• Greiner 150 µL conical well plates for filtrate <30 µL: cat. no. 652270
• Greiner 300 µL plate for samples up to 300 µL: cat. no. 651201
• Greiner 700 µL deep well plate for samples up to 500 µL: cat. no. 786201
