Microporous Sample Prep

At every stage of the drug discovery process, samples must be isolated, purified and prepared prior to testing.

Automation-compatible MultiScreen Filter Plates are used in a range of applications. Membranes and plastic are optimized for use in applications including sample cleanup prior to instrument analysis, removal of cellular debris, extraction of natural products, and bead cleavage assays.

For general sample prep, protein precipitation assays with solvents, bead cleavage assays, and quantitative filtrate transfer suitable for instrumental analysis, MultiScreen Solvinert filter plates are recommended. Hydrophobic or hydrophilic PTFE membrane is offered to provide low extractables, low binding and high recoveries. Purified samples are suitable for downstream analysis by HPLC and LC/MS/MS. The plates also have a one-piece plate design for extended incubations and are built with solvent-resistant membrane and plastics for broad solvent compatibility.

Total Drug from Plasma by Acetonitrile Precipitation

Seven drugs were tested for percent recovery by acetonitrile plasma precipitation. Plasma stock samples (5 mL) were spiked with 3H labeled drug to a final 5 μM drug concentration followed by 1­hour incubation. Protein was precipitated by the addition of acetonitrile (15 mL) and solution vortexed vigorously. An aliquot (300 μL) was added to each well using one plate per column per drug (n=8) and solution was then vacuum filtered (12“ Hg). Percent recovery was determined using an aliquot (100 μL) of filtrate versus an aliquot of the precipitated stock (cpm filtrate/cpm precipitated stock). Results show that both hydrophilic and hydrophobic MultiScreen Solvinert filter plates have >90% drug recovery.