Microcon^® Centrifugal Filter Units

Microcon® Centrifugal Filter Units The lab standard for sub-microliter volumes - Solute recoveries typically >95%, ideal for dilute (ng/mL to µg/mL range) protein solutions - Patented deadstop for reproducible concentrate volumes - Inverted spin method of concentrate retrieval maximizes recovery - Low-binding Ultracel membrane - Color coding indicates MW cut-off - Convenient sample storage of filtrate or concentrated sample in standard microfuge collection tube - Use in standard 1.5 mL tube fixed-angle rotors

Applications
- Concentrating and purifying proteins, antibodies and nucleic acids (alternative to EtOH precipitation)
- Desalting and buffer exchange
- Removal of primers, linkers and unincorporated label


Typical Protein Recovery

Retentate Recovery (%) Solute (Concentration) Nominal MW 3 10 30 50 100 Bovine IgG Fraction II (1 mg/mL) 156,000 95 95 95 95 95 Bovine Serum Albumin (1 mg/mL) 67,000 95 95 95 90 50 Ovalbumin (1 mg/mL) 45,000 95 95 95 90 10 α-Chymotrypsinogen (1 mg/mL) 25,000 95 95 95 80 — Cytochrome c (0.25 mg/mL) 12,400 95 95 90 10 —

Specifications    (return to top)

Volumes Maximum Initial Sample Volume, mL 0.5 Typical Final Concentrate Volume, µL 5–15 Centrifugal Force* Fixed-angle microfuge rotor w/maximum 14,000 x g Materials Membrane Ultracel regenerated cellulose Device Sample reservoir: polypropylene; Sample vial: polycarbonate Membrane Support Base Acetal O-ring Medical grade silicone rubber Dimensions Active Membrane Area, cm2 0.32 Diameter, mm 12.3 Length, in concentration mode, mm 45 *500 x g for DNA/RNA applications with Microcon YM-100 Filter Unit