Immobilon Transfer Membranes, Sandwiches, and Blotting Filter Paper

Polyvinylidene fluoride (PVDF) for superior western, dot, and slot blots

Millipore offers three different Immobilon PVDF membranes, each optimized for different protein blotting applications. We also now offer blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper.

Immobilon membranes provide a number of advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Membrane

   • 0.45 µm pore size
   • Recommended for most western blotting, especially proteins >20 kDa
   • Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Blotting Sandwiches

   • When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper.

Blotting Filter Paper

   • Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-P^SQ Membrane

   • 0.2 µm pore size and a large internal structure
   • Higher protein adsorption and sequencing yields than other
membranes
   • Recommended for blotting proteins <20 kDa
   • Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane

   • The first transfer membrane optimized for fluorescence applications
   • Extremely low background improves sensitivity of all fluorescence detection protocols
   • Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
   • Ideal for multiplexing and chemifluorescence applications

Performance

Rapid Immunodetection Protocol with Immobilon-P Membrane

Immobilon-P^SQ Membrane Prevents Blow-through

Immobilon-FL Membrane is Optimized for Fluorescence

Specifications (return to top)