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Millipore’s Detection Reagents for Western Blotting

Millipore’s Detection Reagents for Western Blotting

Millipore’s Western Blot Detection reagents are designed to make Western detection of proteins simple and cost effective by giving you the highest level of sensitivity with the least amount of background in a chemiluminescent reagent system.

Spray and Glow

The Visualizer™ Spray & Glow™ system is a light-emitting non-radioactive method for detecting immobilized specific antigens in chemiluminescent Western blots through HRP-labeled antibodies. Spray & Glow is comparable in sensitivity to an everyday “workhorse” ECL detection reagent, suitable for normal to high abundance proteins. As a plus, Spray & Glow is easy to use and requires no mixing or pipetting!

Its low background, high signal-to-noise ratio make it ideal for your research. As you may know, low background blots are considered higher quality data that are easier to analyze and more likely to be accepted for publication.

Other Advantages for Your Research:

       • Economical for high abundance proteins
       • Drop-in to existing protocols
       • Substrate and enhancer in a single spray bottle
       • Only single solution ECL system on the market
       • Low list price
       • Compatible with PVDF and NC

Immobilion® Western HRP and AP Chemiluminescent Substrates

Millipore’s HRP and AP substrates excel in all Western and dot/slot/spot blotting applications. In addition, they are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

In addition, they give you high sensitivity over a broad detection range at a much lower cost than other chemiluminescent substrates. This combination of versatility and value makes them the only substrates you need for your Western blotting experiments!

Please see ordering information below to decide what Millipore products are right for your Western blotting research.


Ordering Information  (return to top)

Please select 5 or fewer Items to compare.
Visualizer™ Spray & Glow™ ECL Western Blotting Detection System

Compare Catalogue No. Description  
17-373 Visualizer™ Spray & Glow™ ECL Western Blotting Detection System » Add to Cart
17-373SP Visualizer™ Spray & Glow™ ECL Western Blotting Detection System » Add to Cart
Immobilon Western Chemiluminescent HRP Substrate

Compare Catalogue No. Description Reagents (Volumes) Membrane Coverage, cm2  
WBKLS0020 Immobilon Western HRP Substrate, 20 mL Luminol (10 mL); Peroxide solution (10 mL) 200 » Add to Cart
WBKLS0050 Immobilon Western HRP Substrate, 50 mL Luminol (25 mL); Peroxide solution (25 mL) 500 » Add to Cart
WBKLS0100 Immobilon Western HRP Substrate, 100 mL Luminol (50 mL); Peroxide solution (50 mL) 1000 » Add to Cart
WBKLS0500 Immobilon Western HRP Substrate, 500 mL Luminol (250 mL); Peroxide solution (250 mL) 5000 » Add to Cart
Immobilon Western AP Substrate

Compare Catalogue No. Description Membrane Coverage, cm2  
WBKDS0025 Immobilon Western AP Substrate, 25 mL 500 » Add to Cart
WBKDS0100 Immobilon Western AP Substrate, 100 mL 2000 » Add to Cart