PCR Cleanup Filter Plates
PCR Filter Plate Advantages:
- Automation compatible, fast,
high-throughput solution
- No centrifugation or precipitation
- Excellent capacity, purity and recovery
Millipore's PCR filter plates are fast, automatable
solutions for high-throughput PCR purification. Available in 96- and 384-well
formats as well as micro 96-well format for small volume PCR product
purification, MultiScreen PCR plates provide high purity and high recovery with
a much shorter protocol and fewer handling steps than other methods.
The plates use a size-exclusion membrane and vacuum filtration to provide a
one-step protocol for excellent results. No centrifugation or precipitation
steps are required. This membrane technology also allows for all sample
recovery from the top of the plate to enhance compatibility with liquid
handling systems.
MultiScreen PCRµ96 and PC384
filter plates provide high recovery on even the smallest miniaturized
reactions. Working volumes of <150 µL conserve reaction reagents and save
money. For larger reaction volumes (>150 µL), use MultiScreen PCR96
96-well filter plate.
Protocol
)
| 1. | Load PCR reactions. |
| 2. | Filter with Millipore vacuum manifold for 5–10 minutes or until wells are dry. |
| 3. | Add water or buffer to each well. Agitate by shaking or pipetting. Retrieve purified samples by aspiration. |
Selection Guidelines
| Product name | Reaction volume |
Number of samples |
Mode of operation |
Recommended applications |
Features |
| MultiScreen PCRµ96 Filter Plate | 1–150 µL | 96 | Vacuum | Sequencing Genotyping Microarray |
• Highly concentrated final sample • Reaction miniaturization |
| MultiScreen PCR96 Filter Plate | 150–300 µL | 96 | Vacuum | Microarray | • Volumetric capacity |
| MultiScreen PCR384 Filter Plate | 1–100 µL | 384 | Vacuum | Sequencing Genotyping Microarray |
• Highly concentrated final sample • Reaction miniaturization • Ultra-high throughput |
Performance
Get high reproducibility with MultiScreen PCR96 Filter Plate
)
Agarose gel showing reproducibility of recovery of a 500 bp PCR product with MultiScreen PCR96 filter plate.
Long Sequencing Reads
)
Representative electropherogram from a 301 bp PCR product purified using MultiScreen PCR96 filter plate (Thermo Sequenase® II dye terminator).
Suitable for Microarrays
)
Enlarged section of a 19,299 element microarray (image reproduced with permission from Dr. John Quakenbush, The Institute for Genomic Research). High quality microarrays can be manufactured using PCR products purified with MultiScreen PCR products. Reference: Hegde, P., R. Qi, K. Abernathy, C. Gay, S. Dhaarp, R. Gaspard, J.E. Hughes, E. Snesrud, N. Lee and J. Quakenbush. 2000. A concise guide to cDNA microarray analysis. BioTechniques 29: 548–562.
Multiscreen PCR384 Filter Plate
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