Bead-based Assays

Bead-based assays are an essential tool in the secondary screening process, covering applications such as receptor/ligand studies, enzyme assays, and immunological screens. In addition to secondary screening assays, they are used in combinatorial chemistry applications, ADME/Tox, and general sample prep prior to HPLC, mass spectrometry, and DNA sequencing.

MultiScreen plates are available in a wide range of membranes and plastics making them the ideal tool for any application requiring a solid support for reactions and separations to take place. MultiScreen Solvinert plates have been specifically developed for compatibility with the solvents needed for combinatorial chemistry bead cleavage steps. MultiScreen plates are also available with low binding membranes for immunoassays.

All MultiScreen plates have individual wells that eliminate cross-talk and allow repeated filtrations and incubations. Particular assays may require specialized membranes or plastics, depending on the reactants and detection methods chosen. Details are included in the plate selection guide, .

Using the MultiScreen Column Loader, a variety of soft gel, ion exchange, and reverse phase resins can be loaded into the plate, 96 wells at a time, turning a MultiScreen plate into 96 individual mini-columns.

Plates can also be used as reaction vessels for applications utilizing beads as end-point capture mechanisms or for multiplexing applications using Luminex LabMAP™ technology.

When working with solvents, MultiScreen Solvinert filter plates are recommended for broadest chemical compatibility.

• Fast, efficient washes
  
• High bead recovery
  
• Extended incubation