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Amicon® Ultra-4 Centrifugal Filter Units

Amicon® Ultra-4 Centrifugal Filter Units

The premier device for concentrating 1–4 mL
- High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
- High retentate recovery of >90%
- Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)
- Heat-sealed membrane minimizes downstream extractables
- 100% integrity tested for reliable performance
- Convenient sample monitoring with translucent housing and volume gradations
- Direct pipettor sample access eliminates processing step to recover concentrate
- High concentration factors of 80–100X



Applications
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates
- Concentration of dilute or pre-purified proteins from column eluents
- Desalting and buffer exchange



Typical Spin Times to Filtrate Volume


Spin conditions: 4000 xg swinging bucket rotor at 25 °C, 4 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).




Typical Protein Recovery    (return to top)

Membrane
NMWL, kDa
Protein Solute
Protein Retentate
Volume, mL
Protein Retentate
Recovery, %
3
Cytochrome c, 12.4 kDa
(0.25 mg/mL)
0.19
98.7
10
Cytochrome c, 12.4 kDa
(0.25 mg/mL)
0.20
95
30
BSA, 67 kDa
(1 mg/mL)
0.20
95
50
BSA, 67 kDa
(1 mg/mL)
0.08
94
100
IgG, 156 kDa
(1 mg/mL)
0.34
91
4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).



Specifications    (return to top)

Volumes
Maximum Initial Sample Volume, mL*
4
Typical Final Concentrate Volume, µL
50
Centrifugal Force
Swinging bucket rotor: 4,000 x g; Fixed angle rotor: 7,500 x g for 3, 10, 30, and 50K; 5,000 x g for 100K
Materials
Membrane
Ultracel regenerated cellulose
Device
Housing: styrene/butadiene; Tube: polypropylene; Cap and liner: polyethylene
Dimensions
Active Membrane Area, cm2
3
Diameter, mm
22.7
Length, mm
124
*Except for 23° fixed angle rotor: 3.5 mL



References
http://Concentration of Legionella pneumophila urinary antigen (J Clin Micro 42:9 (4410))


Ordering Information  (return to top)

Select up to 5 products to compare them side by side

Compare Catalogue No. Product Name NMWL, kDa Qty/Pk  
UFC800308 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-3 membrane 3 8 » Add to Cart
UFC800324 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-3 membrane 3 24 » Add to Cart
UFC800396 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-3 membrane 3 96 » Add to Cart
UFC801008 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane 10 8 » Add to Cart
UFC801024 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane 10 24 » Add to Cart
UFC801096 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-10 membrane 10 96 » Add to Cart
UFC803008 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane 30 8 » Add to Cart
UFC803024 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane 30 24 » Add to Cart
UFC803096 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-30 membrane 30 96 » Add to Cart
UFC805008 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-50 membrane 50 8 » Add to Cart
UFC805024 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-50 membrane 50 24 » Add to Cart
UFC805096 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-50 membrane 50 96 » Add to Cart
UFC810008 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-100 membrane 100 8 » Add to Cart
UFC810024 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-100 membrane 100 24 » Add to Cart
UFC810096 Amicon Ultra-4 Centrifugal Filter Unit with Ultracel-100 membrane 100 96 » Add to Cart
Amicon Ultra-4 Filter Units are assembled with centrifuge tubes and caps.
The Amicon Ultra-4 with Ultracel-10 membrane is CE marked.