Amicon^® Ultra-4 Centrifugal Filter Units

Amicon® Ultra-4 Centrifugal Filter Units The premier device for concentrating 1–4 mL - High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs - High retentate recovery of >90% - Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes) - Heat-sealed membrane minimizes downstream extractables - 100% integrity tested for reliable performance - Convenient sample monitoring with translucent housing and volume gradations - Direct pipettor sample access eliminates processing step to recover concentrate - High concentration factors of 80–100X

Applications
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates
- Concentration of dilute or pre-purified proteins from column eluents
- Desalting and buffer exchange



Typical Spin Times to Filtrate Volume

Spin conditions: 4000 xg swinging bucket rotor at 25 °C, 4 mL sample. 3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery    (return to top)

Membrane NMWL, kDa Protein Solute Protein Retentate Volume, mL Protein Retentate Recovery, % 3 Cytochrome c, 12.4 kDa (0.25 mg/mL) 0.19 98.7 10 Cytochrome c, 12.4 kDa (0.25 mg/mL) 0.20 95 30 BSA, 67 kDa (1 mg/mL) 0.20 95 50 BSA, 67 kDa (1 mg/mL) 0.08 94 100 IgG, 156 kDa (1 mg/mL) 0.34 91 4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).

Specifications    (return to top)

Volumes Maximum Initial Sample Volume, mL* 4 Typical Final Concentrate Volume, µL 50 Centrifugal Force Swinging bucket rotor: 4,000 x g; Fixed angle rotor: 7,500 x g for 3, 10, 30, and 50K; 5,000 x g for 100K Materials Membrane Ultracel regenerated cellulose Device Housing: styrene/butadiene; Tube: polypropylene; Cap and liner: polyethylene Dimensions Active Membrane Area, cm2 3 Diameter, mm 22.7 Length, mm 124 *Except for 23° fixed angle rotor: 3.5 mL

References
http://Concentration of Legionella pneumophila urinary antigen (J Clin Micro 42:9 (4410))