Amicon® Ultra-4 Centrifugal Filter Units

The premier device for concentrating 1–4 mL

   • High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
   • High retentate recovery of >90%
   • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 5 minutes)
   • Heat-sealed membrane minimizes downstream extractables
   • 100% integrity tested for reliable performance
   • Convenient sample monitoring with translucent housing and volume gradations
   • Direct pipette sample access eliminates processing step to recover concentrate
   • High concentration factors of 80–100X

Applications

   • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
   • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLC
   • Concentration of dilute or pre-purified proteins from column eluents
   • Desalting, buffer exchange and protein dialysis
   • Size exclusion and protein fractionation

Typical Spin Times to Filtrate Volume

Spin conditions: 4000 xg swinging bucket rotor at 25 °C, 4 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery (return to top)

Specifications (return to top)