Tools for Detection of Serine and/or Threonine Phosphorylation
Overview:
Approximately 400 of the 518 kinases in the human genome are serine/threonine specific protein kinases and approximately 40 serine/threonine specific protein phosphatases are encoded by the human genome. These enzymes are among the most studied biological molecules in both basic research as well as drug discovery laboratories. Given the involvement of these kinases in all aspects of cell signaling, it is not surprising to find that serine/threonine kinases are instrumental in many critical disorders including many cancers, diabetes, and Alzheimer’s disease. As a result, many drug discovery programs are specifically targeting serine/threonine kinases.
The new monoclonal Anti-phosphoserine, clone 4A4 allows for the greatest detection of serine phosphorylation, irrespective of surrounding amino acids on a wide variety of substrates. 4A4 is proven to work with high affinity in a wide variety of applications including western blot, immunofluorescence, IHC, flow cytometry, ELISA, and more. Additionally, in profiling studies, 4A4 detected more serine phosphorylated substrates in multiple cell lysates than any other Anti-phosphoserine monoclonal antibody tested. As many substrates and kinases are phosphorylated on serine residues, this antibody has a very broad range of use.
