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Receptor Binding Assays
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Ordering Information
Technical Library
Receptor Binding Assays
MultiScreen
HTS
96- and 384-well filter plates for separating free from bound, labeled ligand
Receptor-binding assays are a critical component in lead indentification and later lead characterization processes. They are used to characterize most known drug targets and typically use filter-based separations technology to obtain necessary bound vs. free fractions for assay validation. For sensitivity and specificity, radiolabeled known drugs are used in competitive binding assays. The assay is designed as a competitive inhibition assay using the radiolabeled known drug/ligand receptor interaction to screen chemical or natural product libraries for more effective NCEs (new chemical entities).
These quantitative binding parameter determinations indicate the minimal effective drug concentrations. MultiScreen
HTS
plates for quantitative binding parameters provide a more accurate and reliable alternative to homogeneous assays. They are widely used in high throughput screening campaigns and provide a reliable platform that incorporates a range of glass fiber filters to retain the receptor and bound ligand fraction. Operation by vacuum manifold allows for more convenient characterizations since the bound fractions are easily collected from the top of the plate.
The improved plate design of MultiScreen
HTS
automation-compatible filter plates facilitates use with gripper arms and is compatible with microplate scintillation counters.
*NEW*
MultiScreen
HTS
+ Hi Flow filter plates: improve washing efficiency and reduce data variation.
MultiScreen Harvest plates are also available to withstand the numerous washes and batch pretreatment (PEI) required in cell harvest protocols.
- Highly sensitive and specific radiometric assays
- Compatible with liquid scintillation cocktail
- Improved automation compatibility with MultiScreen
HTS
filter plates
Protocol
1.
Add cells, membrane, or tissue to MultiScreen Plate
2.
Incubate with labeled ligand
3.
Collect receptor/ligand complex on filter membrane. Wash and count. If "free vs. bound" study, collect filtrate into 96-well plate
Performance
Optimized Design for High Quality Results
384-well Displacement Binding of Human Muscarinic M1 Receptor
96-well Displacement Binding of Human Muscarinic M1 Receptor
Radioligand binding displacement binding assays were performed with a constant radiolabeled scopolamine concentration (0.6 nM) and serial dilutions of unlabelled pirenzipine as compared to a control binding experiment without unlabelled pirenzipine (% of Control). Left: Displacement binding done with 4.38 µg receptor preparation in 100 µL using MutliScreen
HTS
384-well filter plate. Results presented are from three separate experiments each performed in triplicate wells. Right: Displacement binding done with 8.75 µg receptor preparation in 200 µL using MutliScreen 96-well filter plate. Relative affinity values (IC50) were determined by fitting displacement binding inhibition values by non-linear regression using Prism
data software. All data points are the average of triplicate experiments. Filter plates were dried prior to the addition of Supermix
(10 µL in 384-well plates and 50 µL in 96-well plates). Counting was done in a MicroBeta
®
Trilux. NOTE: All counting is done with underdrain on for both 96- and 384-well plates.
Ordering Information
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Select up to 5 products to compare them side by side
96-well MultiScreen
HTS
Plates with Durapore Membrane (PVDF)
Compare
Catalogue No.
Product Name
Filter Pore Size, µm
Qty/Pk
MSGVN2B50
MultiScreen
HTS
-GV Plate
0.22
50
» Add to Cart
MSHVN4B50
MultiScreen
HTS
-HV Plate
0.45
50
» Add to Cart
MSDVN6B50
MultiScreen
HTS
-DV Plate
0.65
50
» Add to Cart
384-well MultiScreen
HTS
Plates with Durapore Membrane (PVDF)
Compare
Catalogue No.
Product Name
Filter Pore Size, µm
Qty/Pk
MZHVN0W10
MultiScreen
HTS
-HV Plate
0.45
10
» Add to Cart
MZHVN0W50
MultiScreen
HTS
-HV Plate
0.45
50
» Add to Cart
96-well MultiScreen
HTS
Plates with Glass Fiber Filter
Compare
Catalogue No.
Product Name
Filter Pore Size, µm
Qty/Pk
MSFBN6B10
MultiScreen
HTS
-FB Plate
Glass fiber FB, 1.0 + 0.65 durapore
10
» Add to Cart
MSFBN6B50
MultiScreen
HTS
-FB Plate
Glass fiber FB, 1.0 + 0.65 durapore
50
» Add to Cart
MSFBNXB50
MultiScreen
HTS
+ Hi Flow FB Plate
Glass fiber FB, 1.0 supported by polyester mesh
50
» Add to Cart
MSFCN6B10
MultiScreen
HTS
-FC Plate
Glass fiber FC, 1.2 + 0.65 durapore
10
» Add to Cart
MSFCN6B50
MultiScreen
HTS
-FC Plate
Glass fiber FC, 1.2 + 0.65 durapore
50
» Add to Cart
MSFCNXB50
MultiScreen
HTS
+ Hi Flow FC Plate
Glass fiber FC, 1.2µm supported by polyester mesh
50
» Add to Cart
384-well MultiScreen
HTS
Plates with Glass Fiber Filter
Compare
Catalogue No.
Product Name
Filter Pore Size, µm
Qty/Pk
MZFBN0W10
MultiScreen
HTS
-FB Plate
Glass fiber FB, 1.0 supported by polyester mesh
10
» Add to Cart
MZFBN0W50
MultiScreen
HTS
-FB Plate
Glass fiber FB, 1.0 supported by polyester mesh
50
» Add to Cart
MZFCN0W10
MultiScreen
HTS
-FC Plate
Glass fiber FC, 1.2 supported by polyester mesh
10
» Add to Cart
MZFCN0W50
MultiScreen
HTS
-FC Plate
Glass fiber FC, 1.2 supported by polyester mesh
50
» Add to Cart
MultiScreen Harvest Plates
Compare
Catalogue No.
Product Name
Filter Pore Size, µm
Qty/Pk
MAHFB1H60
MultiScreen Harvest Plate
1
60
» Add to Cart
MAHFC1H60
MultiScreen Harvest Plate
1.2
60
» Add to Cart
Accessories
Compare
Catalogue No.
Product Name
Qty/Pk
MSVMHTS00
MultiScreen
HTS
Vacuum Manifold
1
» Add to Cart
MAVM0960R
MultiScreen Vacuum Manifold
1
» Add to Cart
MATAH0P00
MultiScreen Sealing Tape, opaque
100
» Add to Cart
MATAHCL00
MultiScreen Sealing Tape, clear
100
» Add to Cart
MSTPCWH50
Packard Top Count Adapter for MultiScreen
HTS
96-well Filter Plates
50
» Add to Cart