Scepter 2.0 Handheld Automated Cell Counter

For more detailed Scepter information, visit the Scepter Technology webpage.

The Scepter 2.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.

The Scepter cell counter’s screen displays:

• Cell concentration
  
• Average cell size
  
• Average cell volume
  
• Histogram of size or volume distribution

This system is intended for research use only and has been tested with cell types representative of those in use today. Visit www.millipore.com/scepter for sample histograms, performance data, a video demonstration, and testimonials.

The Scepter Histogram:

“A New Window into your Cell Culture”

• Monitor cell health
  
• Check the quality of your culture
  
• Ensure sample-to-sample consistency
  
• Gate your histogram to count only the population that interests you

How the Scepter cytometer delivers cell health information

The Scepter cell counter counts any particle that passes through the orifice within the cell diameter range and will display a histogram of size distribution. Exclude aberrantly large or small cells or debris from the count by manual or automatic gating, to obtain information on the population of interest. Shifts in the histogram may reflect changing culture conditions, and these shifts can be monitored to assess cell culture quality and consistency.

The Scepter Software:

• 72 histograms can be saved to the Scepter device.
  
• Upload data to your computer using the USB cable provided.
  
• Perform additional data manipulations by exporting data to Microsoft Excel.

Scepter Performance:

• The Scepter cytometer counts cells or particles between 8 and 25 μm.
  
• Samples should be diluted within the Scepter operating range (10,000-500,000 cells/mL).
  
• A sample size of 100 μl is recommended.