Luminata Western HRP Substrates
Formulated for optimal results.
The new family of Luminata Western HRP substrates are a family of premixed, ready-to-use chemiluminescent reagents for the detection of HRP-based Westerns. Pour directly onto the Western membrane without worrying about pipetting error. The Luminata™ Classico, Crescendo and Forte substrates cover a broad range of sensitivities.
Advantages
- Premixed for convenience and
to avoid cross contamination of the reagents.
- Broad range of
sensitivities to satisfy all detection needs.
- Consistent
results with less pipetting error.
- Stable at 4 °C or room
temperature so you can store it on your own bench.
- Best
detection performance relative to competition.
- Compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.
Three different sensitivities of Luminata substrates meet all detection needs
)
Detection of GAPDH in 10 μg (Lane 1), 5 μg (Lane 2), 2.5 μg (Lane 3), and 1.2 μg (Lane 4) of A431 lysate. Blots were treated with the indicated detection substrate and exposed to x-ray film for 5 minutes. Luminata Forte substrate is equivalent to Immobilon Western Chemiluminescent HRP Substrate (WBKLS0500, Millipore).
Premixed Luminata substrate signals stay strong even after long term storage
)
Luminata substrates, either in premixed form or in their
parent luminol and peroxide components, were used to detect signals on
immunoblots containing various amounts of A431 lysate probed with anti-GAPDH
(MAB374, Millipore).
Luminata substrates produced similar signals in their
premixed format as in their separated 2 component system.
Signals remained
robust even after 6 months storage. (Storage study conducted under accelerated
aging methodology.)
Luminata™ HRP Chemiluminescence Detection Reagents
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