MultiScreen® Filter Plate with Ultracel®-10 Membrane

High Throughput UF Technology

MultiScreen filter plate with Ultracel-10 membrane provides a new method for high throughput sample prep. The
ultrafiltration-based filter plate is designed for automation-compatible sample purification, concentration and desalting of biological solutions, as well as protein removal from samples prior to instrument analysis.

The 96-well MultiScreen filter plate incorporates Ultracel-10 10,000 kDa nominal molecular weight limit regenerated cellulose ultrafiltration membrane for low-binding, high recovery results. It is designed for use with centrifugation and is compatible with standard microtiter plates, instrumentation, and liquid handling equipment.

Reliable

MultiScreen filter plate with Ultracel-10 membrane is designed for low protein binding and high protein retention. The assay-grade plates meet stringent manufacturing release specifications and are lot tested for protein retention for >95% retention of cytochrome c (12,500 Daltons). They also offer high well-to-well uniformity on performance.

Versatile Operation

MultiScreen filter plate with Ultracel-10 membrane allows for processing and collection of sample volumes from 50 to 500 µL. They are compatible with a range of standard receiver 96-well microtiter plates including 300 µL, 700 µL deep well and 150 µL conical well.

MultiScreen filter plate with Ultracel-10 membrane is ideal for applications including protein concentration and desalting prior to validation or screening assays. They are able to remove large molecular weight proteins prior to instrument analysis.

Performance

Different protein solutes with an initial sample volume of 300 µL were spun at 2000 x g. Ultrafiltrate volumes are lower for samples with higher protein concentrations such as serum.

Typical Protein Recovery

Membrane NMWL, kDa	Protein Solute	Typical Retention
10,000	Cytochrome c 12,500 kDa (1 mg/mL)	95%
10,000	BSA 67,000 kDa (1 mg/mL)	99%
30 minute spin time at 2000 x g with a final concentration volume of 130 µL.

Specifications

Volumes
Filter Plate Capacity, mL	0.5
Working Sample Volume Capacity	Limited to receiver plate. Compatible with standard 96-well microtiter plates (300 µL, 700 µL deep well, and 150 µL conical u-bottom).*
Centrifugal Force	Maximum 3,000 x g
Materials
Membrane	Ultracel regenerated cellulose
Device	Polyolefin
Dimensions
Length, mm	128
Width, mm	85.2
Height, mm	14.2
*Recommended receiver plates sold separately. See "Required Equipment" for details.

Required Equipment (not included)

Centrifuge capable of a minimum of 2000 x g with a swinging bucket rotor and 96-well plate carrier
Pipetters or robotic liquid handlers for transferring 10 µL, 25 µL and 300 µL volumes
96-well microtiter plate receiver tray

Recommended Receiver Plates

Millipore Solvinert Collection Plate for UV analysis: cat. no. MSCP NPP 00
Millipore Deep Well Collection Block: cat. no. MDCP N2M 50
Greiner 150 µL conical well plates for filtrate <30 µL: cat. no. 652270
Greiner 300 µL plate for samples up to 300 µL: cat. no. 651201
Greiner 700 µL deep well plate for samples up to 500 µL: cat. no. 786201

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Compare	Catalogue No.	Product Name	Qty/Pk
	MAUF01010	MultiScreen Filter Plate with Ultracel-10 Membrane	10

Includes MultiScreen filter plates and lids. Receiver plate must be purchased separately.