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Amicon Ultra-2 mL Centrifugal Filters for Protein Purification and Concentration

With their vertical membrane design, Amicon Ultra 2 mL filters deliver the highest recovery with shortest spin times
 

  • Concentrates 2 mL down to 15-70 µl
  • 98% sample recovery
  • Fast processing time - 10 to 30 minute spin times
  • Engineered dead stop
  • Reverse spin capability provides consistent recoveries

Applications

  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates
  • Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC
  • Desalting, buffer exchange and protein dialysis
  • Size exclusion and protein fractionation

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Material Supplied

The Amicon Ultra-2 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.

Performance – Protein Concentration

Spin conditions: Swinging bucket rotor, 4,000 × g, room temperature, 2 mL 
starting volume.<br />Protein markers used: Cytochrome c for 3K and 10K, BSA 
for 30K and 50K, and IgG for 100K, n=4.

Spin conditions: Swinging bucket rotor, 4,000 × g, room temperature, 2 mL starting volume.
Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=4.

Spin conditions: 35° fixed angle rotor, 7,500 × g, room temperature, 2 mL 
starting volume.<br />Protein markers used: Cytochrome c for 3K and 10K, BSA 
for 30K and 50K, and IgG for 100K, n=4.

Spin conditions: 35° fixed angle rotor, 7,500 × g, room temperature, 2 mL starting volume.
Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=4.

Protein Retention and Concentrate Recovery

Typical Retention of Protein Markers
Marker
(Concentration)
Molecular
Weight
Device
NMWL
% Retention
Swinging Bucket
Spin Time
(min)
% Retention
Fixed Angle
Spin Time
(min)
Cytochrome c (0.25 mg/mL) 12,400 3K > 99 60 > 99 60
Cytochrome c (0.25 mg/mL) 12,400 10K > 98 40 > 99 20
BSA (1 mg/mL) 67,000 30K > 99 20 > 99 15
BSA (1 mg/mL) 67,000 50K > 96 15 > 97 10
IgG (1 mg/mL) 156,000 100K > 98 30 > 98 20
Spin Conditions: Swinging bucket rotor, 4,000 x g, or 35º fixed angle rotor, 7,500 x g, 2 mL starting volume, room temperature, n=4.

Desalting or Diafiltration

Desalting, buffer exchange or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the Amicon Ultra-2 device by concentrating the sample, then reconstituting the concentrate to the original sample volume with any desired solvent. The process of "washing out" can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced. See example below.

Amicon Ultra-2 mL Centrifugal Filters for DNA Purification and Concentration


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