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Elispot MultiScreenHTS Filter Plates and Antibody Pairs

 

Improved Spot Recovery and Spot Definition

Elispot assays are used for a range of applications to monitor immune responses and immunological response patterns, and can also be used to identify antigenic peptides and create vaccination strategies. Validated Elispot assays are highly reliable, reproducible and predictive. They can successfully identify antigenspecific T or B cells on a single cell level with results that closely mirror in vivo conditions and eliminate the need for long-term in vitro cell cultures or the addition of exogenous cytokines.

New: 8-Well Strips now available for processing less than 96 samples at one time.

MultiScreenHTS filter plates are available with validated Immobilon®-P (PVDF) for superior results. Plates with HA (mixed cellulose esters) are also available. Both provide membranes with dense uniform pore structure to promote antibody binding and increase sensitivity for better, sharper spot definition.

  • Validated membranes
  • Uniform spot morphology
  • Easy membrane access
  • Compatible with automation

Protocol

1. Coat membrane with antibodies. Add immune cells and incubate
2. Responding cells produce cytokines. The cytokine of interest is then bound by the antibody
3. Wash to remove cells. Add biotinylated antibodies which bind to the cytokine-antibody complex
4. Add avidin-enzyme conjugate
5. Add enzyme substrate and each responding cell will result in one spot

Uniform and Reproducible Results

Optimized Membrane Gives Consistent Results Even in Corner and Edge Wells

These images represent the number of cells secreting IFN-Ɣ in response to PHA-L stimulation of Human Peripheral Blood Mononuclear Cells (HPBMC). The wells were seeded with 50,000 cells and developed using AEC substrate. The wells were imaged with a CTL Series 5 Analyzer. Typical MultiScreen<sub>HTS</sub>-IP filter plate variability expressed by %CV* is less than 10%. <br />* %CV = (SD/mean)*100<br />Courtesy of Cellular Technology Ltd.

These images represent the number of cells secreting IFN-Ɣ in response to PHA-L stimulation of Human Peripheral Blood Mononuclear Cells (HPBMC). The wells were seeded with 50,000 cells and developed using AEC substrate. The wells were imaged with a CTL Series 5 Analyzer. Typical MultiScreenHTS-IP filter plate variability expressed by %CV* is less than 10%.
* %CV = (SD/mean)*100
Courtesy of Cellular Technology Ltd.

3D well image rendering provides valuable insights in per cell cytokine productivity.

3D well image rendering provides valuable insights in per cell cytokine productivity.

Elispot Antibody Pairs

EMD Millipore's Elispot antibody sets come as wel-validated matched pairs of monoclonals to human or mouse inmmunology targets with and enzymatic detection. Each set has sufficient reagents for five, 96-well plates and includes:

  • Monoclonal Capture Antibody
  • Monoclonal Detection Antibody
  • Streptavidin Alkaline Phosphatase

Photo courtesy of Carl Zeiss MicroImaging, Inc.

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