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Millicell 24-well Cell Culture Plate

 

Ideal system for a range of cell-based assays

The Millicell 24-well Cell Culture Assembly is designed to support cell attachment, growth and differentiation for many cell applications. The plate assembly is used for cell growth as well as analysis and can be used manually or with automated cell seeding, feeding and washing systems.

Both a single-well feeding and 24-well receiver plate are available for use.

Patented cell-culture plate improves cell growth and analysis

The new 24-well Millicell Cell Culture plate assembly is designed for maximum user convenience and reliability. The plate features basolateral access ports at each well to allow for media addition without disassembling the cell culture plate and receiver tray. Additionally, the cell culture plate has feet to raise and protect the cell monolayer if the receiver tray is removed. Other features like raised well edges for improved tape sealing and large font labeling provide added user convenience.

The 24-well feeder tray incorporates a unique tear-drop well design to reduce the incidence of air bubbles that can form under the membrane during plate assembly. This feature ensures that cell monolayers are getting proper nutrients.

If a single-well tray is required, the Millicell assembly features a single-well tray with baffles to reduce media leakage and contamination.

Design improves cell culture assays and analysis

Performance

In-plate differentiation of embryonic stem cells and phenotype analysis

Neuron differentiation of embryonic stem cells in 
Millicell 24 1 µm PET filter plates. Murine embryonic stem cells were formed 
into suspended embryoid bodies (EBs) then transferred to 1 µm PET Millicell 24 
for attachment and differentiation. Neural differentiation after retinoic acid 
treatment of attached EBs was confirmed by anti-neurofilament 
immunofluoresence. (Insert: Inverted phase contrast imaging through membrane of 
live EBs in media.)

Neuron differentiation of embryonic stem cells in Millicell 24 1 µm PET filter plates. Murine embryonic stem cells were formed into suspended embryoid bodies (EBs) then transferred to 1 µm PET Millicell 24 for attachment and differentiation. Neural differentiation after retinoic acid treatment of attached EBs was confirmed by anti-neurofilament immunofluoresence. (Insert: Inverted phase contrast imaging through membrane of live EBs in media.)

Demonstrated transport and efflux studies with integral differentiated Caco-2 monolayers

Differentiation of 21-day Caco-2 monolayer by efflux pump expression. 
Anti-MDR1 immunohistochemistry confirms p-glycoprotein (MDR1) expression of 
Caco-2 monolayer grown in Millicell 24 with 1 µm PET membrane.

Differentiation of 21-day Caco-2 monolayer by efflux pump expression. Anti-MDR1 immunohistochemistry confirms p-glycoprotein (MDR1) expression of Caco-2 monolayer grown in Millicell 24 with 1 µm PET membrane.


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