Cryopreserved HepaRG™ Cells

HepaRG™ cells are terminally differentiated human hepatocellular carcinoma cells that reproducibly express the activities of transporters and drug metabolizing enzymes. Functionally equivalent to primary human hepatocytes, they facilitate studies of uptake, metabolism, and disposition of drug candidates. EMD Millipore is the exclusive supplier of Biopredic’s original, widely cited HepaRG™ cells, manufactured by them to the highest quality standards.

Advantages: Quality, Convenience and Value

• Respond and function like primary human hepatocytes across a range of applications
  
• Do not require daily medium changes, are reproducible and can be stored until required
  
• Can be used for diverse studies at a lower overall cost than human primary hepatocytes

Dependable alternative to human hepatocytes

Human hepatocytes are often deemed crucial for in vitro drug metabolism and toxicity studies. The supply of fresh human hepatocytes is unpredictable, and the material is often of poor quality, subject to genetic and environmental variability and costly. Even less ideal are the alternatives, including rat hepatocytes, recombinant non-human or non-hepatocyte cell lines, and cryopreserved human hepatocytes, all of which lose the functional activities of fresh, human hepatocytes.

HepaRG™ cells are therefore an innovative, practical solution for reproducible studies of hepatotoxicity and metabolism.

• Cryopreserved HepaRG™ Cells

1 mL in vial with ≥8 x 106 viable cells
One vial will fully seed one 96-well plate (72,000 cells/well) or 16 wells of one 24-well plate (480,000 cells/well)

• HepaRG™ Thawing/Plating Medium Supplement

12.5 mL
When combined with 100mL of base medium, sufficient to thaw four vials and plate into four plates

• HepaRG™ Tox Medium Supplement

12.5 mL
When combined with 100mL of base medium, sufficient for ten medium changes

• HepaRG™ Induction Medium Supplement

2.6 mL
When combined with 100mL of base medium, sufficient for ten medium changes for induction studies

• HepaRG™ Serum Free Induction Medium Supplement

0.6 mL
When combined with 100 mL of base medium, sufficient for ten medium changes for induction studies

• HepaRG™ Culture Medium Supplement

14.0 mL
When combined with 100 mL of base medium, sufficient for twelve medium changes for studies where prolonged cell life or enhanced metabolic function is required

• View HepaRG™ Cell Applications Document
  
• View HepaRG™ Cell Bibliography Document