Overview of the SNAP i.d.® 2.0 Protein Detection System
Developed to meet the needs of our Western blotting
customers, the SNAP i.d.® 2.0 system produces blots of a very high quality
every time – in record time! Unique vacuum-driven technology and a built-in
flow distributor actively drive reagents through the membrane, ensuring even
distribution. Two blot holder sizes accommodates mini Mini (7.5 x 8.4 cm) or
midi Midi (8.5 x 13.5 cm) (W x L) ) size gels and two blot holders can be run
in parallel. Thus, you can quickly optimize conditions and greatly increase
your protein detection throughput.
Typically, researchers lack the time to optimize their blotting protocols. By shortening the time required for blocking, washing and antibody incubations to 30 minutes, the SNAP i.d.® 2.0 system allows you to optimize your immunodetection conditions for the highest quality results.
The ordering tables on the next tab show how you can easily obtain the SNAP i.d.® 2.0 system and its components. We look forward to hearing about your research results once you have used your new tool for Western blotting!
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SNAP i.d.® 2.0 Hardware and Accessories
Your SNAP i.d.® 2.0 system includes everything you need to
get started, including blot holders, two independent blot holder frames,
antibody collection tray and blot roller. In addition, we provide you with an
integrated vacuum regulator to ensure consistent vacuum pressure without the
need for an external regulator.
Other useful items include: Chemical duty pumps (115 V/60 Hz) or (220 V/50 Hz), a one-liter Vacuum filtering flask, Silicone No. 8 perforated stoppers (5 to a pack) and stainless steel forceps.
Western Blotting Reagents
Our Western Blotting Detection Kits are designed to make
the Western detection of proteins easy and economical. We offer an excellent
selection of reagents, including Bløk® noise cancelling reagents designed to
minimize background in Western blots, increasing sensitivity for superior
detection of even low-abundance proteins. Bløk® reagents are protein-free,
which reduces non-specific binding, especially when you are using
phosphoantibodies or avidin-biotin systems.
We also offer Immobilion® Western HRP and AP Chemiluminescent Substrates that cost less than the competition and provide higher sensitivity over a broad detection range. This combination of versatility and value makes them the only substrates you need for your Western blotting experiments.
Other offerings include the Reblot™ Plus Western Blot Recycling Kit, the product of choice for quick and effective removal of antibodies from Western blots without significantly affecting the immobilized proteins. And, our ChemiBlot™ Molecular Weight Markers estimate molecular weight on SDS-PAGE and use as a positive control for Western blot.
Our PVDF transfer membranes are available in three varieties, each optimized for different protein blotting applications: Immobilion®-P, Immobilion®-PSQ, and Immobilion®-FL. All have exceptional handling characteristics and are compatible with a variety of detection chemistries. We also offer blotting sandwiches with pre-cut sheets of membrane and blotting filter paper interleaved for your convenience.