Overview of the SNAP i.d.® Protein Detection System

Developed to meet the needs of our Western blotting customers, the SNAP i.d. system produces blots of a very high quality every time – in record time! Unique vacuum-driven technology and a built-in flow distributor actively drive reagents through the membrane, ensuring even distribution. Three different sizes of blot holders accommodate up to three blots each, and two blot holders can be run in parallel. Thus, you can process up to six blots at the same time, quickly optimize conditions and greatly increase your protein detection throughput.

Typically, researchers lack the time to optimize their blotting protocols. By shortening the time required for blocking, washing and antibody incubations to 30 minutes, the SNAP i.d. system allows you to optimize your immunodetection conditions for the highest quality results.

The ordering tables below show how you can easily obtain the SNAP i.d. system and its components. We look forward to hearing about your research results once you have used your new tool for Western blotting!

SNAP i.d. Hardware and Accessories

Your SNAP i.d. System includes a base with two independent blot holder stations. Each side can run separately. In addition, Millipore provides you with an integrated vacuum regulator to ensure consistent vacuum pressure without the need for an external regulator.

Blot holders are available in single, double, and triple sizes. Our antibody collection tray is another useful accessory. You can also order a blot roller.

Other useful items include: Chemical duty pumps (115 V/60 Hz) or (220 V/50 Hz), a one-liter Vacuum filtering flask, Silicone No. 8 perforated stoppers (5 to a pack) and stainless steel forceps.

Western Blotting Reagents

Millipore’s Western Blot Detection Kits are designed to make the Western detection of proteins easy and economical. We offer an excellent selection of reagents, including Bløk noise cancelling reagents designed to minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins. Bløk reagents are protein-free, which reduces non-specific binding, especially when you are using phosphoantibodies or avidin-biotin systems.

We also offer Immobilion® Western HRP and AP Chemiluminescent Substrates that cost less than the competition and provide higher sensitivity over a broad detection range. This combination of versatility and value makes them the only substrates you need for your Western blotting experiments.

Other offerings include our Visualizer™ Spray & Glow ECL reagent that is suitable for normal to high abundance proteins. Its spray-on format is easy to use with no mixing or pipeting needed. We also offer Re-Blot Plus Western Blot Recycling Kits that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins.

In addition, you may wish to purchase ChemiBlot Molecular Weight Markers so you can estimate molecular weight on SDS-PAGE and use as a positive control for Western blot.

Blotting Membranes

Millipore offers three varieties of PVDF transfer membranes, each optimized for different protein blotting applications: Immobilion®-P, Immobilion-PSQ, and Immobilion-FL. All have exceptional handling characteristics and are compatible with a variety of detection chemistries. We also offer blotting sandwiches with pre-cut sheets of membrane and blotting filter paper interleaved for your convenience.