Predictive models for oral absorption
Increasing drug candidate predictability earlier in the discovery pipeline using non-cell-based assays can improve candidate throughput during the ADME stage. Non-cell-based permeability assays are predictive models for oral absorption of development candidates.
MultiScreen Permeability, MultiScreen-IP, and MultiScreen Acceptor plates support artificial membrane strategies for predicting passive transport.
Membranes have uniform pore structure and density for reliable transport
• Individual wells minimize candidate cross-talk
• Compatibility with automated liquid handling systems increases throughput
Permeability Assay
The MultiScreen Permeability plate has a polycarbonate membrane, which supports a hexane/hexadecane artificial layer for permeability assays.1
PAMPA Assay
The MultiScreen-IP plate has a hydrophobic PVDF membrane, which supports a lipid bilayer for PAMPA asays.2,3
MultiScreen Acceptor Plate
The MultiScreen Acceptor plate can be used with either plate to capture passively transported compounds. Constructed of 100% PTFE, the plate can improve LC/MS results depending on the charge of the small molecules being evaluated.
Materials
Permeability plate: polystyrene plate with polycarbonate membrane
MultiScreen-IP plate: acrylic plate with hydrophobic PVDF membrane
Acceptor plate: entirely PTFE
Liquid Handling Systems
Tecan Genesis, Packard MultiPROBE®
References
1. JMC, July 2000, F. Wohnsland, B. Faller, JMC0201.pdf, High-Throughput Permeability pH Profile and High-Throughput Alkane/Water log P with Artificial Membranes, Publication RP1013ENUS
2. JMC 1998, Vol.41, No.7, pp 1007–1010, M. Kansy, F. Senner, K. Gubernator, Physicochemical High Throughput Screening: Parallel Artificial Membrane Permeation Assay in the Description of Passive Absorption Processes, Publication RP1011ENUS
3. JBS, 2001, Vol. 6, No. 3, K. Sugano, H. Hamada, M. Machida, and H. Ushio, pp 189–196, High Throughput Prediction of Absorption: Improvement of the Composition of the Lipid Solution Used Parallel Artificial Membrane Permeation Assay, Publication RP1014ENUS
