Millipore - Amicon Ultra-15 Centrifugal Filter Units

The premier device for concentrating <15 mL

● High recovery Ultracel regenerated cellulose membrane in a range of molecular weight cut-offs
● High retentate recovery of >90%
● Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes)
● Heat-sealed membrane minimizes downstream extractables
● 100% integrity tested for reliable performance
● Convenient sample monitoring with translucent housing and volume gradations
● Direct pipettor sample access eliminates processing step to recover concentrate
● High concentration factors of 80–100X

Applications

● Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
● Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS
● Concentration of dilute or pre-purified proteins from column eluents
● Desalting, buffer exchange and protein dialysis
● Size exclusion and protein fractionation

Typical Spin Times to Filtrate Volume

Spin conditions: 4000 xg swinging bucket rotor at 25°C, 15 mL sample.
3K, 10K: Cytochrome c (0.25 mg/mL); 30K, 50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).

Typical Protein Recovery (return to top)

Membrane NMWL, kDa	Protein Solute	Protein Retentate Volume, mL	Protein Retentate Recovery, %
3	Cytochrome c, 12.4 kDa (0.25 mg/mL)	0.294	97.5
10	Cytochrome c, 12.4 kDa (0.25 mg/mL)	0.24	93.03
30	BSA, 67 kDa (1 mg/mL)	0.30	97.94
50	BSA, 67 kDa (1 mg/mL)	0.17	93.32
100	IgG, 156 kDa (1 mg/mL)	0.28	89.03
15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min).

Specifications (return to top)

Volumes
Maximum Initial Sample Volume, mL*	15
Typical Final Concentrate Volume, µL	200
Centrifugal Force	Swinging bucket rotor: 4,000 x g; Fixed angle rotor (35°): 5,000 x g
Materials
Membrane	Ultracel regenerated cellulose
Device	Housing: styrene/butadiene; Tube: polypropylene; Cap and liner: polyethylene
Dimensions
Active Membrane Area, cm²	7.6
Diameter, mm	29.7
Filtrate tube or vial (centrifuge tube)
Length, mm (with cap and device)	122
Length, mm (without cap and device)	115
Filter unit
Diameter, mm	29.6
Length, mm	72.0
*Except for 35° fixed angle rotor: 12 mL

Amicon Ultra-15 Centrifugal Filter Unit with Ultracel-10 membrane is CE marked.

比较	目录编号	产品名称	NMWL，kDa	数量/包装
	UFC900308	装有 Ultracel-3 超滤膜的 Amicon Ultra-15 离心超滤管	3	8	» 添加到购物车
	UFC900324	装有 Ultracel-3 超滤膜的 Amicon Ultra-15 离心超滤管	3	24	» 添加到购物车
	UFC900396	装有 Ultracel-3 超滤膜的 Amicon Ultra-15 离心超滤管	3	96	» 添加到购物车
	UFC901008	装有 Ultracel-10 超滤膜的 Amicon Ultra-15 离心超滤管	10	8	» 添加到购物车
	UFC901024	装有 Ultracel-10 超滤膜的 Amicon Ultra-15 离心超滤管	10	24	» 添加到购物车
	UFC901096	装有 Ultracel-10 超滤膜的 Amicon Ultra-15 离心超滤管	10	96	» 添加到购物车
	UFC903008	装有 Ultracel-30 超滤膜的 Amicon Ultra-15 离心超滤管	30	8	» 添加到购物车
	UFC903024	装有 Ultracel-30 超滤膜的 Amicon Ultra-15 离心超滤管	30	24	» 添加到购物车
	UFC903096	装有 Ultracel-30 超滤膜的 Amicon Ultra-15 离心超滤管	30	96	» 添加到购物车
	UFC905008	装有 Ultracel-50 超滤膜的 Amicon Ultra-15 离心超滤管	50	8	» 添加到购物车
	UFC905024	装有 Ultracel-50 超滤膜的 Amicon Ultra-15 离心超滤管	50	24	» 添加到购物车
	UFC905096	装有 Ultracel-50 超滤膜的 Amicon Ultra-15 离心超滤管	50	96	» 添加到购物车
	UFC910008	装有 Ultracel-100 超滤膜的 Amicon Ultra-15 离心超滤管	100	8	» 添加到购物车
	UFC910024	装有 Ultracel-100 超滤膜的 Amicon Ultra-15 离心超滤管	100	24	» 添加到购物车
	UFC910096	装有 Ultracel-100 超滤膜的 Amicon Ultra-15 离心超滤管	100	96	» 添加到购物车