PureProteome™ Magnetic Beads

ELIMINATE VARIABILITY. MAXIMIZE RECOVERY.

A paramagnetic, porous silica surface. Scanning electron microscopy shows closeups of Millipore's magnetic beads.

Complete Recovery with Reproducible Results

Millipore’s new PureProteome magnetic beads ensure the rapid and reproducible isolation of proteins. Unlike conventional methods that require centrifugation to pellet the beads then careful aspiration to avoid sample loss, PureProteome magnetic beads are isolated using a magnetic rack. This allows for the total removal of buffers for complete recovery of beads no sample dilution. You eliminate variability while maximizing recovery.

Advantages

   • High capacity: More than 6x the binding capacity of competitive magnetic beads.
   • Consistent Results: Total removal of buffers with no sample loss
   • Fast processing time: Beads are immobilized in seconds
   • Economical: Up to half the price of competitive magnetic beads

Protein A and Protein G beads are ideal for immunoprecipitation and serum depletion assays. Use the Nickel Magnetic Bead system for purifying His-tagged proteins.

Comparison of traditional and magnetic bead purification methods

PureProteome Protein A and Protein G Magnetic Beads for Immunodepletion and Antibody Purification

With a capacity 6x higher then competitive beads, PureProteome Protein A and Protein G magnetic beads provide the greatest percentage of immunoglobulins (IgG) depletion from serum samples with low non-specific binding. Get up to 8X greater recovery in half the time.

Comparison of Protein Bead Effectiveness

Table 1.: Rabbit Serum (50 uL) diluted with PBS was incubated with Protein A and Protein G magnetic beads per manufacturer’s instructions. An ELISA assay was used to determine the percentage of IgG depleted and the results were normalized by the volume of slurry used per reaction.

Depletion Profile: Rabbit Serum (50 µL) diluted with PBS was incubated with Protein G magnetic beads per manufacturer’s instructions. The depleted rabbit serum samples were separated by SDS-PAGE and the gel stained with Coomassie Blue using Millipore and competitor magnetic beads. Lane 1: input material, lanes 2, 4, 6, 8: depleted samples, lanes 3, 5, 7, 9: eluted samples.

PureProteome Nickel Magnetic Beads for His-tagged Protein Purification

Use PureProteome Nickel Magnetic Beads to get high yield, high purity preps of you're His-tagged recombinant protein.

As shown in the Coomassie-stained gel below, Millipore's nickel magnetic bead system can give you greater purity of protein, without compromising yield.