Amicon Ultra-4 and -15 Centrifugal Filter Units – 10,000 NMWL – For in vitro diagnostic use (CE)
- Amicon Ultra-4* and -15*
Centrifugal Filter Units are designed for in vitro diagnostic use and intended
for concentrating serum, urine, cerebrospinal fluid and other body fluids prior
to analysis.
- The premier devices for concentrating 1 – 4mL or <15 mL
- High recovery Ultracel regenerated cellulose membrane in a range of
molecular weight cut-offs
- High retentate recovery of >90%
- Vertical membrane reduces concentration polarization for ultra-fast spin
times (as fast as 10–15 minutes)
- Heat-sealed membrane minimizes
downstream extractables
- 100% integrity tested for reliable
performance
- Convenient sample monitoring with translucent housing and
volume gradations
- Direct pipettor sample access eliminates processing
step to recover concentrate
- High concentration factors of 80–100X
* The Amicon Ultra-4 and Amicon Ultra-15 with Ultracel-10 membrane are CE marked.
Applications
- Concentration of biological samples
containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture
extracts or cell lysates
- Concentration of dilute or pre-purified
proteins from column eluents
- Desalting and buffer exchange
Performance data - Amicon Ultra-4 Centrifugal Filter Unit
Typical Spin Times to Filtrate Volume
)
Spin conditions: 4000 xg swinging bucket rotor at 25 °
C, 4 mL sample.
3K,
10K: Cytochrome c (0.25 mg/mL); 30K,
50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery
| Membrane NMWL, kDa |
Protein Solute | Protein Retentate Volume, mL |
Protein Retentate Recovery, % |
| 10 | Cytochrome c, 12.4 kDa (0.25 mg/mL) |
0.20 | 95 |
| 4 mL starting volume in a fixed angle rotor at 7,500 x g at 25 °C. Spin times: 3K (30 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min). | |||
Performance data - Amicon Ultra-15 Centrifugal Filter Unit
Typical Spin Times to Filtrate Volume
)
Spin conditions: 4000 xg swinging bucket rotor at 25°
C, 15 mL sample.
3K,
10K: Cytochrome c (0.25 mg/mL); 30K,
50K: BSA (1 mg/mL); 100K: IgG (1 mg/mL).
Typical Protein Recovery
| Membrane NMWL, kDa |
Protein Solute | Protein Retentate Volume, mL |
Protein Retentate Recovery, % |
| 10 | Cytochrome c, 12.4 kDa (0.25 mg/mL) |
0.24 | 93.03 |
| 15 mL starting volume in swinging bucket rotor at 4000 x g at 25 °C. Spin times: 3K (40 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min). | |||
Amicon Ultra filter units process 15 mL samples as fast as 10 15 minutes
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