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Amicon Ultra-0.5 mL Centrifugal Filters for Protein Purification and Concentration

With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin times

       • Concentrates 500 µL down to 15 µL
       • Concentration factor 25 x to 30 x
       • High sample recovery (greater than 90% of dilute starting solution)
       • Fast processing time - 10 to 30 minutes
       • Consistent recoveries thanks to the reverse spin capability
       • Reverse spin capability provides consistent recoveries

Amicon05_ReverseSpin-71[83660-ALL].jpg

Applications

       • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
       • Purification of macromolecular components found in tissue culture extracts or cell lysates
       • Primer removal, PCR clean up, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC
       • Desalting, buffer exchange and protein dialysis
       • Size exclusion and protein fractionation

Material Supplied

The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.

Performance – Protein Concentration

Amicon05_new-71[84695-ALL].jpg

Spin conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein marker used: Cytochrome c, n=6.

Typical Concentrate Volume/Concentration Factor vs. Spin Time

Concentrate Volume / Concentration Factor
3K device10K device30K device50K device100K device
Spin time (min)Conc. Volume (µL)Conc. Factor (x)Conc. Volume (µL)Conc. Factor (x)Conc. Volume (µL)Conc. Factor (x)Conc. Volume (µL)Conc. Factor (x)Conc. Volume (µL)Conc. Factor (x)
5215266742122818589
1011443514232220251926
158062222192717301533
206282024173015331336
3048101531153215361141
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume. Protein markers used: Cytochrome c for 3K and 10K, BSA for 30K and 50K, and IgG for 100K, n=12. Shaded volumes were used for the calculation of protein recovery in the Typical Concentrate Recovery table.

Typical Retention of Protein Markers

Marker/ConcentrationMolecular WeightDevice NMWL% RetentionSpin Time (min)
α-Chymotrypsinogen (1 mg/mL)25,0003K> 9530
Cytochrome c (0.25 mg/mL)12,400> 9530
Vitamin B-12 (0.2 mg/mL)1,350< 4230
α-Chymotrypsinogen (1 mg/mL)25,00010K> 9515
Cytochrome c (0.25 mg/mL)12,400> 9515
Vitamin B-12 (0.2 mg/mL)1,350< 4215
BSA (1 mg/mL)67,00030K> 9510
Ovalbumin (1 mg/mL)45,000> 9510
Cytrochorme c (0.25 mg/mL)12,400< 3510
BSA (1 mg/mL)67,00050K> 9510
Ovalbumin (1 mg/mL)45,000~ 4010
Cytrochorme c (0.25 mg/mL)12,400< 2010
Thyroglobulin (0.5 mg/mL)677,000100K> 9510
IgG (1 mg/mL)156,000> 9510
Ovalbumin (1 mg/mL)45,000< 3010
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, n=12.

Typical Concentrate Recovery

Marker/ConcentrationMolecular WeightDevice NMWLSpin Time (min)Concentrate Volume (µL)Concentration Factor (x)Concentrate Recovery (%)
Cytochrome c (0.25 mg/mL)12,4003K30481098
Cytochrome c (0.25 mg/mL)12,40010K15222293
BSA (1 mg/mL)67,00030K10232297
BSA (1 mg/mL)67,00050K10202592
IgG (1 mg/mL)156,000100K10192692
Spin Conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, n=12. The shaded volumes were taken from the Typical Concentrate Volume/Concentration Factor vs. Spin Time table.

Desalting or Diafiltration

Desalting, buffer exchange or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the Amicon Ultra-0.5 device by concentrating the sample, then reconstituting the concentrate to the original sample volume with any desired solvent. The process of "washing out" can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced. See example below.

Amicon05_Desalting-71[83650-ALL].jpg

Specifications (return to top)

Volumes
Maximum initial sample volume, µL500
Typical final concentration volume, µL15 – 20
Recommended relative centrifugal force14,000 x g for concentration spin
1,000 x g for reverse spin
Maximum relative centrifugal force15,000 x g
Active membrane area, cm21
Hold up volume, µL< 1
Materials
Filter deviceCopolymer styrene/butadiene
MembraneMillipore Ultracell low-binding regenerated cellulose
Collection tubePolypropylene
The ultrafiltration membranes in Amicon Ultra-0.5 mL devices contain trace amounts of the following:Amicon Ultra 3K devices: glycerine
Amicon Ultra 10K device: triethylene glycol (TEG)
If these materials interfere with analysis, pre-rinse the device with buffer or Milli-Q water. If interference continues, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q water.
Dimensions
Filter device, mm (in.)Diameter: 9.4 (0.37)
Length: 29.6 (1.17)
Tube, mm (in.)Diameter: 10.8
Length: 43.0 (1.69)
Filter device and tube, mm (in.)Length (concentration mode; device in tube): 49.9
Length (reverse spin; device upside down in tube): 37.3 (1.47)

Chemical Compatibility of Amicon Ultra Filters

AcidsConcentrationAcidsConcentration
Acetic acid ≤ 50%*Phosphoric acid ≤ 30%
Formic acid ≤ 5%*Sulfamic acid≤ 3%
Hydrochloric acid ≤ 1.0 MSulfuric acid ≤ 3%
Lactic acid ≤ 50%Trichloroacetic acid (TCA)≤ 10%*
Nitric acid ≤ 10%Trifluoroacetic acid (TFA)≤ 30%*
AlkalisConcentrationAlkalisConcentration
Ammonium hydroxide ≤ 10%Sodium hydroxide ≤ 0.5 M
AlcoholsConcentrationAlcoholsConcentration
n-Butanol ≤ 70%Isopropanol ≤ 70%
Ethanol ≤ 70%Methanol ≤ 60%
DetergentsConcentrationDetergentsConcentration
Alconox® detergent ≤ 1%Lubrol® PX detergent ≤ 0.1%
CHAPS detergent≤ 0.1%Nonidet™ P-40 surfactant ≤ 2%
Sodium deoxycholate≤ 5%Triton® X-100 surfactant≤ 0.1%
Sodium dodecyl sulfate (SDS)≤ 0.1%Tween® 20 surfactant≤ 0.1%
Terg-A-Zyme® detergent ≤ 1%
Organic solventsConcentrationOrganic solventsConcentration
Acetonenot recommendedEthyl acetatenot recommended
Acetonitrile ≤ 20%Formaldehyde ≤ 5%
Benzenenot recommendedPyridinenot recommended
Carbon tetrachloridenot recommendedTetrahydrofurannot recommended
Chloroformnot recommendedToluenenot recommended
Dimethyl sulfoxide (DMSO)≤ 5%*
MiscellaneousConcentrationMiscellaneousConcentration
Ammonium sulfate Saturated Phenol≤ 1%
Diethyl pyrocarbonate ≤ 0.2%Phosphate buffer (pH 8.2)≤ 1 M
Dithiothreitol (DTT)≤ 0.1 MPolyethylene glycol≤ 10%
Glycerine≤ 70%Sodium carbonate ≤ 20%
Guanidine HCl ≤ 6 MTris buffer (pH 8.2)≤ 1 M
Imidazole≤ 100 mMUrea ≤ 8 M
Mercaptoethanol≤ 0.1 M
* Contact with this chemical may cause materials to leach out of the component parts. Solvent blanks are recommended to determine whether leachables represent potential assay interferences.


Información pedidos  (volver a la parte superior)

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UFC500396 Amicon Ultra-0.5, Ultracel-3 Membrane, 3 kDa 3 96 » Añadir al pedido
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UFC5010BK Amicon Ultra-0.5, Ultracel-10 Membrane, 10 kDa 10 500 » Añadir al pedido
UFC503008 Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa 30 8 » Añadir al pedido
UFC503024 Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa 30 24 » Añadir al pedido
UFC503096 Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa 30 96 » Añadir al pedido
UFC5030BK Amicon Ultra-0.5, Ultracel-30 Membrane, 30 kDa 30 500 » Añadir al pedido
UFC505008 Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa 50 8 » Añadir al pedido
UFC505024 Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa 50 24 » Añadir al pedido
UFC505096 Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa 50 96 » Añadir al pedido
UFC5050BK Amicon Ultra-0.5, Ultracel-50 Membrane, 50 kDa 50 500 » Añadir al pedido
UFC510008 Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa 100 8 » Añadir al pedido
UFC510024 Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa 100 24 » Añadir al pedido
UFC510096 Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa 100 96 » Añadir al pedido
UFC5100BK Amicon Ultra-0.5, Ultracel-100 Membrane, 100 kDa 100 500 » Añadir al pedido
Accessories

Comparar Referencia Descripción Cantidad/envase  
UFC50VL96 Amicon Ultra 0.5ml Vials 96 » Añadir al pedido