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Amicon Ultra-0.5 mL Centrifugal Filters for DNA Purification and Concentration

With their vertical membrane design, Amicon Ultra 0.5 mL filters deliver great performance and lower spin times

       • Concentrates 500 µL down to 15 µL
       • Concentration factor 25 x to 30 x
       • High sample recovery (greater than 90% of dilute starting solution)
       • Fast processing time - 10 to 30 minutes
       • Consistent recoveries thanks to the reverse spin capability
       • Reverse spin capability provides consistent recoveries

Amicon05_ReverseSpin-71[83660-ALL].jpg

Applications

       • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
       • Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromelecular labels from a reaction mix, and protein removal prior to HPLC
       • Desalting, buffer exchange, and protein dialysis

Performance – DNA Concentration

The Amicon Ultra-0.5 device provides the best balance between recovery and spin time for double-stranded DNA for base pairs ranging from 137 to 1159. To achieve maximum PCR product recovery and primer removal with primers greater than 20 bases, one or two additional spins with Tris-EDTA (TE) buffer are recommended.

Typical Recovery of Nucleotides from Amicon Ultra-0.5 30K Devices
PCR Product (base pairs)PCR Primer (bases) PCR Recovery (%)PCR Primer Removal (%)TE Washes (number)
13710≥95≥900
20≥90≥851
48≥90≥752
30110≥90≥900
20≥85≥901
48≥90≥802
64810≥95≥900
20≥90≥901
48≥95≥902
115910≥90≥900
20≥90≥951
48≥95≥952
Spin conditions: 40º fixed angle rotor, 14,000 x g, room temperature, 500 µL starting volume, 20-30 µL final volume, 10 minute spin, n=12.

Desalting or Diafiltration

Desalting, buffer exchange or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the Amicon Ultra-0.5 device by concentrating the sample, then reconstituting the concentrate to the original sample volume with any desired solvent. The process of "washing out" can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced. See example below.

Amicon05_Desalting-71[83650-ALL].jpg

Specifications (return to top)

Volumes
Maximum initial sample volume, µL500
Typical final concentration volume, µL15 – 20
Recommended relative centrifugal force14,000 x g for concentration spin
1,000 x g for reverse spin
Maximum relative centrifugal force15,000 x g
Active membrane area, cm21
Hold up volume, µL< 1
Materials
Filter deviceCopolymer styrene/butadiene
MembraneMillipore Ultracell low-binding regenerated cellulose
Collection tubePolypropylene
The ultrafiltration membranes in Amicon Ultra-0.5 mL devices contain trace amounts of the following:Amicon Ultra 3K devices: glycerine
Amicon Ultra 10K device: triethylene glycol (TEG)
If these materials interfere with analysis, pre-rinse the device with buffer or Milli-Q water. If interference continues, rinse with 0.1 N NaOH followed by a second spin of buffer or Milli-Q water.
Dimensions
Filter device, mm (in.)Diameter: 9.4 (0.37)
Length: 29.6 (1.17)
Tube, mm (in.)Diameter: 10.8
Length: 43.0 (1.69)
Filter device and tube, mm (in.)Length (concentration mode; device in tube): 49.9
Length (reverse spin; device upside down in tube): 37.3 (1.47)

Chemical Compatibility of Amicon Ultra Filters

AcidsConcentrationAcidsConcentration
Acetic acid ≤ 50%*Phosphoric acid ≤ 30%
Formic acid ≤ 5%*Sulfamic acid≤ 3%
Hydrochloric acid ≤ 1.0 MSulfuric acid ≤ 3%
Lactic acid ≤ 50%Trichloroacetic acid (TCA)≤ 10%*
Nitric acid ≤ 10%Trifluoroacetic acid (TFA)≤ 30%*
AlkalisConcentrationAlkalisConcentration
Ammonium hydroxide ≤ 10%Sodium hydroxide ≤ 0.5 M
AlcoholsConcentrationAlcoholsConcentration
n-Butanol ≤ 70%Isopropanol ≤ 70%
Ethanol ≤ 70%Methanol ≤ 60%
DetergentsConcentrationDetergentsConcentration
Alconox® detergent ≤ 1%Lubrol® PX detergent ≤ 0.1%
CHAPS detergent≤ 0.1%Nonidet™ P-40 surfactant ≤ 2%
Sodium deoxycholate≤ 5%Triton® X-100 surfactant≤ 0.1%
Sodium dodecyl sulfate (SDS)≤ 0.1%Tween® 20 surfactant≤ 0.1%
Terg-A-Zyme® detergent ≤ 1%
Organic solventsConcentrationOrganic solventsConcentration
Acetonenot recommendedEthyl acetatenot recommended
Acetonitrile ≤ 20%Formaldehyde ≤ 5%
Benzenenot recommendedPyridinenot recommended
Carbon tetrachloridenot recommendedTetrahydrofurannot recommended
Chloroformnot recommendedToluenenot recommended
Dimethyl sulfoxide (DMSO)≤ 5%*
MiscellaneousConcentrationMiscellaneousConcentration
Ammonium sulfate Saturated Phenol≤ 1%
Diethyl pyrocarbonate ≤ 0.2%Phosphate buffer (pH 8.2)≤ 1 M
Dithiothreitol (DTT)≤ 0.1 MPolyethylene glycol≤ 10%
Glycerine≤ 70%Sodium carbonate ≤ 20%
Guanidine HCl ≤ 6 MTris buffer (pH 8.2)≤ 1 M
Imidazole≤ 100 mMUrea ≤ 8 M
Mercaptoethanol≤ 0.1 M
* Contact with this chemical may cause materials to leach out of the component parts. Solvent blanks are recommended to determine whether leachables represent potential assay interferences.


Informações para Pedidos  (retornar ao início)

Por favor selecione 05 (cinco) ou menos itens para comparar.

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