Scepter 2.0 Handheld Automated Cell Counter
For more detailed Scepter information, visit the Scepter Technology webpage.
The Scepter 2.0 handheld automated cell counter provides a fast and convenient method for counting cells and particles and is compatible with both the 40 and 60uM sensor. The system employs the Coulter principle in a miniaturized, handheld, format enabling rapid cell counting; what used to take 10 minutes now takes less than 30 seconds. The user prepares a dilution of the cell culture of interest and uses the Scepter cell counter to aspirate a sample of this dilution into the Scepter sensor.
The Scepter cell counter’s screen displays:
- Cell concentration
- Average cell size
- Average cell volume
- Histogram of size or volume distribution
This system is intended for research use only and has been tested with cell types representative of those in use today. Visit www.millipore.com/scepter for sample histograms, performance data, a video demonstration, and testimonials.
The Scepter Histogram:
“A New Window into your Cell Culture”
- Monitor cell health
the quality of your culture
- Ensure sample-to-sample consistency
- Gate your histogram to count only the population that interests you
How the Scepter cytometer delivers cell health information
The Scepter cell counter counts any particle that passes through the orifice within the cell diameter range and will display a histogram of size distribution. Exclude aberrantly large or small cells or debris from the count by manual or automatic gating, to obtain information on the population of interest. Shifts in the histogram may reflect changing culture conditions, and these shifts can be monitored to assess cell culture quality and consistency.
The Scepter Software:
- 72 histograms can be saved to the
- Upload data to your computer using the USB cable
- Perform additional data manipulations by exporting data to Microsoft Excel.
- The Scepter cytometer counts cells
or particles between 8 and 25 μm.
- Samples should be diluted within
the Scepter operating range (10,000-500,000 cells/mL).
- A sample size of 100 μl is recommended.