Amicon Ultra-2 mL Pre-Launch Centrifugal Filters for DNA Purification and Concentration

• Concentrates 2 mL down to 15-70 µL
  
• 98% sample recovery
  
• Fast processing time - 10 to 30 minute spin times
  
• Engineered dead stop
  
• Reverse spin capability provides consistent recoveries

Pre-launch Device Disclaimer

This pre-launch product is in the final stages of both product and process development, and validation activities are ongoing. The information presented is based on preliminary development results. Device performance in specific applications may be different.

Applications

• Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  
• Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromelecular labels from a reaction mix, and protein removal prior to HPLC
  
• Desalting, buffer exchange, and protein dialysis

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Performance – DNA Concentration

The Amicon Ultra-2 30K device provides the best balance between PCR recovery and PCR primer removal for double-stranded DNA for base pairs ranging from 137 to 1159.

Desalting or Diafiltration

Desalting, buffer exchange or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the Amicon Ultra-2 mL device by concentrating the sample, then reconstituting the concentrate to the original sample volume with any desired solvent. The process of "washing out" can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced. See example below.