ApopNexin™ Apoptosis Detection Kits

Highly sensitive and specific biochemical results
Quantitative results using flow cytometry
Two labeling options

ApopNexin Apoptosis Detection Kits detect the appearance of PS in the outer leaflet of the membrane of the cell. The system uses Annexin V conjugated to either FITC or Biotin to allow for convenient quantitative assays. The counterstain Propidium Iodide is included to differentiate the apoptotic and necrotic cells.

ApopNexin FITC binding can be analyzed by flow cytometry or fluorescence microscopy in less than one hour. It may be used in conjunction with other fluorescent cell surface markers. ApopNexin Biotin can be used for chromogenic or fluorescence detection using a streptavidin conjugate of choice. This versatile approach also allows bicolor or tricolor fluorescence experiments for multiparameter cell surface analysis. Fixation can be performed after Annexin V binding for further analysis of other cellular antigens.

Highly Specific Results

Human peripheral blood lymphocytes induced with an anti-Fas antibody for 3–4 hours and then assayed with ApopNexin FITC and propidium iodide. Results obtained by fluorescence microscopy show that apoptotic cells have ApopNexin FITC bound to the PS on the outer membrane surface (Panel A). As apoptosis progressed, the plasma membrane becomes permeable, and the propidium iodide is able to enter into the cell and bind to cellular DNA (Panels B and C). Necrotic cells are detected by intense propidium iodide staining of the cytoplasm, due to complete disruption of the plasma membrane (Panel D).

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ApopNexin™ Apoptosis Detection Kits

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