ApopTag Peroxidase ISOL Apoptosis Detection Kit
- Novel labeling method clearly differentiates apoptotic from necrotic cells
- Minimizes background through high specificity of in situ labeling
- Reduces the incidence of false positives
The ApopTag ISOL Kit procedure attaches a specific biotinylated, synthetic oligonucleotide to the fragmented DNA in situ, provided that the DNA has a blunt end or a single base overhang on its 3’ end. Either Oligo A (3’ overhang) or Oligo B (blunt end) will bind to an exact complementary, doublestranded DNA end in a reaction catalyzed by T4 DNA Ligase. Then, a streptavidin-peroxidase conjugate is added that binds tightly to the biotin on the oligo. Lastly, the addition of DAB (diaminobenzidine), a chromogenic peroxidase substrate, causes a brown precipitate that can be clearly visualized using brightfield microscopy.
Detection of Apoptotic Cells in Rat Intestine Using the ApopTag ISOL Kit, Protocol One |
ApopTag ISOL Technology |
