Caspa TAG In Situ Apoptosis Detection Kits
- Assay completed in less than 90 minutes Suspension Array Detection
- One-step assay in live cells
- Irreversibly binds to active caspases with high affinity
CaspaTag In Situ Apoptosis Detection Kits detect caspases in living cells undergoing apoptosis. Fluorescently labeled inhibitors of caspase activity irreversibly bind to active caspases, allowing apoptotic cells to be identified by their fluorescence using flow cytometry, fluorescence microscopy or fluorescence reader.
Suspension Array Detection Junkat cells were treated with camptothecin to induce apoptosis. Cells were then labeled with SR-VAD-FMK and Hoechst stain and visualized by fluorescence microscopy. Cells with caspase activity are shown in red (left) while nuclear staining of all cells is shown in blue (right). |
CaspaTag Kits utilize carboxyfluorescein (FAM) or sulforhodamine (SR) labeled fluoromethyl ketone (FMK) inhibitor probes. These probes are derivatives of benzyloxycarbonyl-peptide (caspase recognition sequence)-FMK caspase inhibitors. They enter the cell and irreversibly bind to caspase 3, 7 (DEVD), caspase 8 (LETD), caspase 9 (LEHD) or all caspases (VAD).
CaspSCREEN™ Flow Cytometric
- Fast flow cytometry assay detects all caspase activity
- One-step assay in live cells
- Effective for use in live cells
The CaspSCREEN Flow Cytometric Apoptosis Detection Kit provides a simple and convenient means for detecting active caspases by flow cytometry in non-adherent cells. The assay utilizes a molecule that contains rhodamine 110 linked to two aspartate residues (D2R), a reported substrate for the caspase protease family. The intact substrate is nonfluorescent; however, upon cleavage by caspases, the released rhodamine 110 gives rise to fluorescence that can be measured at Ex = 488 nm and Em = 530 nm. The D2R substrate is more cell-permeable than other fluorometric caspase substrates to effectively and easily detect caspase activity, and therefore, apoptosis.
Detection of Apoptosis by CaspSCREEN Flow Cytometric Analysis Kit |
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