Cell-based Assays
Cell based assays are an invaluable tool in basic research applications and drug screening techniques. The complexity of cell-to-cell and cell-to-extracellular matrix interactions require simple, yet accurate methods to understanding cellular responses and communication under user-defined conditions. Cell based assays are an ideal format for applications ranging from basic research to library mining, lead optimization, and target validation.
- Study cell surface adhesion receptor binding to extracellular matrix (ECM) proteins and the effects of ECM protein involvement in cell movement
- Measure haptotaxis - directed cell movement towards an immobilized ECM protein gradient
- Boyden chamber porous insert membrane is coated on the outside with ECM protein
QCM™ Chemotaxis Cell Migration Assays
- Study cell motility solely in response to a chemical gradient
- Measure chemotaxis - directional cell movement in response to chemical concentration gradients
- Boyden chamber porous insert membrane is uncoated
QCM™ Cell Invasion Assays
- Study cell invasion by cellular degradation of extracellular matrix proteins in response to a chemoattractant located in the outer plate well
- Measure invasion - directional cell migration through the physical penetration of an ECM protein layer
- Boyden chamber porous insert membrane is coated on the inside with ECM protein
CyQuant GR® is registered trademark of Molecular Probes, Inc. The reagent is licensed from Molecular Probes, Inc., and is for use in kits sold by Chemicon International, Inc., for the monitoring of cell invasion and cell migration only.
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Pathways
