Cell Migration
MultiScreen-MIC 96-well Plates
MultiScreen-MIC filter plates are recommended for cell-based screening assays, including migration, invasion, chemotaxis, co-culture, angiogenesis, and transmigration. These 96-well plates incorporate polycarbonate membranes in a variety of pore sizes to support a range of cell types.
The plates can be used with suspension or adherent cell lines, and they support cell growth for co-culture and transmigration assays. Results show that the plates demonstrate high assay consistency with little inter-assay variability. The sterile plates are also shown to support longer incubation times and allow for assay set-up and analysis in the same device. For more information please see www.millipore.com/cellbased.
- Use with adherent or suspension cells
- 3, 5, and 8 µm polycarbonate membrane pore sizes available
- Consistent results
Pore Size Recommendations
| Cell Name | Cell Type | Pore Sizes Used (µm) | Assays Typically Performed |
| MDA-MB-231 | Invasive breast cancer cell line (human) | 5 or 8 | Chemotaxis or invasion assay |
| MCF7 | Non-invasive breast cancer cell line (human) | 5 or 8 | Chemotaxis or invasion assay |
| HT1080 | Invasive breast cancer cell line (human) | 5 or 8 | Chemotaxis or invasion assay |
| NIH3T3 | Non-invasive fibroblast cell line (mouse) | 5 or 8 | Chemotaxis or invasion assay |
| HUVEC | Endothelial cells | 3, 5 or 8 | Chemotaxis, invasion, angiogenesis or transendothelial migration assays |
| HMVEC /HMEC | Endothelial Cells | 3, 5 or 8 | Chemotaxis, invasion, angiogenesis or transendothelial migration assays |
| PMN | Polymorphonuclear neutrophils | 3 | Chemotaxis assays |
| K562 | Human leukemia | 3 | Chemotaxis assays |
| HB124 | Mouse leukemia | 3 | Chemotaxis assays |
Pore size determination is dependent upon cell type. The chart illustrates pore size choices for a selection of cell lines used in Millipore laboratories and by customers for the assays indicated. For more information, contact Millipore Technical Service.
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