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Markers of Developmental Lineages

Following differentiation, pluripotent stem cells give rise to cells of all three embryonic germ layers. Specialized cells from these germ layers constitute the complex assortment of tissues that make up an entire organism. The characterization of differentiated progeny is of particular importance in studies relating to the utilization of pluripotent stem cells to treat degenerative diseases, such as diabetes and chronic heart disease. The use of immunological detection methods has been critical in several studies including the identification of insulin producing cells (Assady et al., 2001, Diabetes) or functional cardiomyocytes (Kehat et al., 2001, J. Invest.) derived from human ES cells. Millipore offers a wide range of reagents for the immunological detection of tissue specific markers, including transcription factors and structural proteins.

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