Eosinophil Isolation Kit

Purify viable and functionally intact eosinophils from peripheral blood
Only 4 mL of EDTA-anticoagulated whole blood is required
Complete isolation is less than 30 minutes

Eosinophils are a rare type of granulocyte that comprises fewer than 2% of normal leukocytes in the peripheral blood. Increased eosinophil counts (eosinophilia) are most often associated with allergic reactions and parasitic infestations, but can also result from other disorders (e.g. skin diseases, autoimmune diseases, asthma, hay fever, Hodgkin’s disease, leukemia). Typically residing in submucosal tissue, eosinophils are recruited to the infection site where they discharge their toxic, granular contents to fight infection.

Millipore’s Eosinophil Isolation Kit is a straightforward method for purifying eosinophils from EDTAanticoagulated blood samples. The peripheral blood is sequentially mixed with calibrated amounts of hypotonic lysis solutions and neutralizing buffers under carefully controlled incubation conditions. The lysate is then subjected to differential centrifugation to pellet the intact eosinophils.

Kit Components

Osmotic Lysis Buffer
Neutralization Buffer
Resuspension Buffer
Eosinophil Counting Stain

Eosinophil Isolation Kit

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