Rescue of ES Cell Lines Using RESGO Culture Medium
RESGRO Culture Medium has the capacity to rescue traditional ES cell lines that have started drifting and either generate low percentage chimeras or have lost germline transmission capability. Differentiated ES cells not visible with traditional ES cell culture medium will become visible with RESGRO medium. After 2 passages, a clear difference is seen between differentiated and undifferentiated ES cells. Cultures can then be subcloned to select the undifferentiated cells.
The selection procedure should be repeated if some differentiation is still present after one subcloning procedure.
Materials and Reagents
- RESGRO Culture Medium
- Millipore cat. nos. SCM001, SCM002
- EmbryoMax ES Cell Qualified L-Glutamine Solution
- Millipore cat. no. TMS-002-C
- Cellulose acetate, PVDF or PES filters
- 70 mm fiberglass prefilter
- Centrifuge • ES Cell Medium
- Incubator, 37°C/5% CO2
- Pipette
- PMEF Feeder cell coated culture plates
- 0.05% Trypsin-0.53mM EDTA -- Millipore cat. no. SM-2002-C
- Water Bath, 37°C
Product News & Releases
- Faster Flow Rates for Sterile Filtration - New Millex® syringe filters with Millipore Express PLUS (PES) membrane
- ReNcell® VM Neural Stem Cell Line Models Parkinson’s Disease - New human disease model shows function of PINK1 gene in neural degeneration
- Millipore Issues New Laboratory Filtration Product Guide
- Millipore Launches Progenitor Cell-Enriched Primary Human Epithelial Cell Cultures
Featured Products
- Fast-Trap Lentivirus Purification and Concentration Kit
- Fast-Trap Adenovirus Purification and Concentration Kit
- EasyCyte Plus Flow Cytometry System
- ECM Cell Culture Optimization Arrays
- ESGRO Complete
- GCTM-5 Antibody, clone GCTM-5
- Guava Viacount Assay
- MilliTrace Constitutive GFP Reporter
- MultiScreenHTS + and MultiScreen Vacuum Manifolds
- ENStem-A hES-derived Human Neural Progenitors