Gene Transfer
Determining the impact of protein function on specific cellular pathways and mechanisms using gene transfer is a key technique for studying specific proteins.
We provide one of the broadest collections of protocols for affecting specific genes. RNAi, the
technology that won the 2006 Nobel Prize in medicine, is one of the newest and easiest methods for
lowering specific gene expression and, in most cases, protein expression. Millipore offers three RNAi-based methods for gene knockdown:
- The short interfering RNAs of the SMARTpools, a pKD line of validated mammalian siRNA expression plasmid
- The AdenoSilence family for adenoviral delivery of RNAi, the newest offering in the gene expression area
- The popular siIMPORTER® Transfection Reagent
Gene Targeting
The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is applied to the cells...
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RNAi Products
Millipore’s siIMPORTER siRNA Transfection Reagent sets a new standard for RNA Interference assays to cell signaling...
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SMARTpool siRNA
SMARTpool siRNA collection consists of at least four individual siRNA duplexes targeted against a specific gene and designed...
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Mammalian Cell Transfection Kit
Transfected cells integrate and express the exogenous DNA at high efficiency, producing stable clones of the specifically altered genotype and phenotype.
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Polybrene Infection/Transfection Reagent
A highly efficient method of gene transfer into mammalian cells is through infection with retroviral vectors. The efficiency of retroviral infection is enhanced significantly, 100 to 1,000 ...
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Dexamethasone Transfection Protocol
Dexamethasone is used to induce glucocorticoid responsive cells. Cells that are transiently or stably transfected with a vector...
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Mammalian Cell Transfection Kit Protocol
Step by step protocol....
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Polybrene Infection/Transfection Reagent Protocol 1
Step by step protocol....
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Polybrene Infection/Transfection Reagent Protocol 2
Step by step protocol....
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Sodium Butyrate Protocol
Sodium butyrate (Millipore cat. no.TR-1008-G) can act to increase the efficiency of transfection and...
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Transient Expression Transfection Kit Protocol
DEAE dextran mediated transfection (Millipore cat. no. S-100) is used for high efficiency, transient expression of cloned genes...
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Knock-down Protocol
Transduction is the process by which the genetic material contained in the virus is transferred to the recipient cell...
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Product News & Releases
- Millipore Issues New Laboratory Filtration Product Guide
- Millipore Launches Progenitor Cell-Enriched Primary Human Epithelial Cell Cultures
- Millipore Launches MILLIPLEX™ MAP
- Millipore Adds to Stem Cell Tested Initiative
Featured Products
- ENStem-A Human Neural Progenitors
- ReNcell Human Neural Progenitors
- HEScGRO Human Embryonic Stem Cell Medium
- Cardiac Stem Cell Isolation Kit
- Mouse Embryonic Stem Cell Neurogenesis Assay
- Cellntec Products for Bladder Research
- Steriflip Filter Units
- Stem Cell Tested Filter Units
- Epidermal Growth Factors

