Gene Transfer

Determining the impact of protein function on specific cellular pathways and mechanisms using gene transfer is a key technique for studying specific proteins.

We provide one of the broadest collections of protocols for affecting specific genes. RNAi, the technology that won the 2006 Nobel Prize in medicine, is one of the newest and easiest methods for lowering specific gene expression and, in most cases, protein expression. Millipore offers three RNAi-based methods for gene knockdown:

• The short interfering RNAs of the SMARTpools, a pKD line of validated mammalian siRNA expression plasmid
• The AdenoSilence family for adenoviral delivery of RNAi, the newest offering in the gene expression area
• The popular siIMPORTER® Transfection Reagent

With these offerings, Millipore leads the way with the most novel and effective products for infecting both growing and primary cells.

Gene Targeting

The Mammalian Cell Transfection Kit is an optimized system suitable for the introduction of DNA into mammalian cells. DNA is applied to the cells...
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Genetic Modification

Viruses are an increasingly valuable tool in bioresearch. A variety of virus-derived vectors have been developed for improved gene transfer in mammalian cells. Highly purified virus is necessary...
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RNAi Products

Millipore’s siIMPORTER siRNA Transfection Reagent sets a new standard for RNA Interference assays to cell signaling...
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SMARTpool siRNA

SMARTpool siRNA collection consists of at least four individual siRNA duplexes targeted against a specific gene and designed...
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Mammalian Cell Transfection Kit

Transfected cells integrate and express the exogenous DNA at high efficiency, producing stable clones of the specifically altered genotype and phenotype.
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Polybrene Infection/Transfection Reagent

A highly efficient method of gene transfer into mammalian cells is through infection with retroviral vectors. The efficiency of retroviral infection is enhanced significantly, 100 to 1,000 ...
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Dexamethasone Transfection Protocol

Dexamethasone is used to induce glucocorticoid responsive cells. Cells that are transiently or stably transfected with a vector...
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Mammalian Cell Transfection Kit Protocol

Step by step protocol....
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Polybrene Infection/Transfection Reagent Protocol 1

Step by step protocol....
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Polybrene Infection/Transfection Reagent Protocol 2

Step by step protocol....
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Sodium Butyrate Protocol

Sodium butyrate (Millipore cat. no.TR-1008-G) can act to increase the efficiency of transfection and...
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Transient Expression Transfection Kit Protocol

DEAE dextran mediated transfection (Millipore cat. no. S-100) is used for high efficiency, transient expression of cloned genes...
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Knock-down Protocol

Transduction is the process by which the genetic material contained in the virus is transferred to the recipient cell...
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