Mammalian Cell Transfection Kit
Introduction of DNA into Mammalian Cells Compenet for Transfection Application
Transfected cells integrate and express the exogenous DNA at high efficiency, producing stable clones of the specifically altered genotype and phenotype.
The Mammalian Cell Transfection Kit provides a protocol and reagents to transfect up to 75 X 100 mm plates.
Kit Components
| 10X HBS | 4 vials | 1.25 mL/vial |
| NaOH Solution | 1 vial | 1.25 mL/vial |
| CaCl2 | 4 vials | 1.25 mL/vial |
| Ultra Pure Water | 1 bottle | 100 mL |
| Phosphate Solution | 1 vial | 1.25 mL/vial |
Concentrations of the reagents in the Mammalian
Cell Transfection Kit are as follows:
| 10X HBS | 119.15 g/L HEPES 163.63 g/L NaCl |
| NaOH Solution | 40.0 g/L |
| CaCl2 | 294.04 g/L |
| Phosphate Solution | 19.318 g/L Sodium Phosphate, monobasic 37.52 g/L Sodium Phosphate, dibasic |
Specifications
- Storage: Store kit at 4°C
- Sterility: All reagents are supplied sterile
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