Multiwell Cell Culture Insert Plate
Millicell 24- and 96-well cell culture insert plates are designed to support cell attachment, growth and differentiation for many cell lines including MDCK and Caco-2. The plates are compatible with automated cell seeding, feeding, and washing systems. Unique features such as the apical assist and the tear-drop well design provide greater convenience and reproducibility than standard multiwell cell culture insert plates.
- Patented plate design improves cell growth and analysis
- Optimized for user convenience
- Automation compatible
Plate Features
Apical Assist Protects Cell Monolayer
A small lip on the inside edge of each well prevents the tip of the pipette from going too far into the apical well and accidentally touching the cell monolayer. (See illustration above.)
Unique Design Prevents Trapped Air
A unique tear-drop well design reduces the chance of air bubbles forming under the filter plate, which can interfere with optimal cell feeding. The singlewell feeder tray has baffles to reduce media leakage and contamination.Ports Provide Easy Basolateral Access
Patented apical and basolateral access ports provide contamination-free access to cell monolayers. They also simplify cell feeding, media changes, and sample analysis. Basolateral access ports are especially effective during transport rate analysis as there is no need to disassemble the assay system to sample basolaterally. Each well and basolateral access hole is aligned to facilitate the use of automated probes.
These automation-compatible plates incorporate a patented design to maintain assay integrity and prevent monolayer disruption, contamination or damage during analysis. The 96-well growth assemblies include a choice of a 96-well or single-well feeder trays. The format is also available in a 24-well design. |
Formats Available
Millicell-24 Cell Culture Insert Plates
Larger Membrane Area Increases Assay Sensitivity
Millicell-24 plates have twice the surface area of other 24-well membrane-based plates, so you can increase cell growth and assay sensitivity.Smaller Liquid Volumes Mean Less Dilution of Transported Material
Millicell-24 plates have a recommended 1:2 ratio between the volumes of liquid in the apical and basolateral chambers, as compared to other 24-well inserts that have up to a 1:6 ratio. The smaller differential in Millicell-24 plates results in less dilution of transported material, higher signal, and greater sensitivity.Additional Features Improve Convenience
Feet on the underside of the growth plate allow you to place it on a sterile surface in the hood while feeding — you do not need to hold the plate. Raised well-edges improve tape sealing and the well-identification information is large and easy to read.Millicell–96 Cell Culture Insert Plates
Complete System for Cell Growth and Experimental Analysis
With this complete system, you can grow, feed, and analyze cells in one membrane-bottom plate. The plate can be used with either a single-well or a 96-well receiver tray for growth. At the time of analysis, the plate is simply transferred to a new 96-well receiver tray for analysis. This streamlined design enhances compatibility with:- Seed-and-feed systems
- Most liquid handlers
- Transepithelial electrical resistance (TEER) measurement systems
Choice of Membranes Lets You Optimize for Your Application
Millicell-24 and Millicell-96 cell culture plates can be used for a wide variety of applications from cell surface receptor assays, to in vitro toxicology, to transport/permeability studies. See the applications guide on page 35 for a complete listing.The plates are available with thin-film polycarbonate (PCF) membrane or polyethylene terephthalate (PET), which is available in a microscopically-transparent 1.0 µm pore size.
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