Millipore - New Cell Biology Products

» More Cell Biology Products
» New Stem Cell Products

Stem Cell Antibodies

Anti-Oct-4 [POU5F1]

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass. The relationship between Oct-4 and pluripotency makes this transcription factor one of the most reliable markers of pluripotent stem cells. This antibody has been validated in flow cytometry, immunocytochemistry (right), and Western blotting applications in both human and mouse samples.

Description	Qty/Pk	Catalogue Number
Anti-Oct4 (POU5F1), clone 7F9.2	100 μg	MAB4419

Anti-BMP-7

Bone morphogenetic protein-7 (BMP-7) is widely expressed during embryonic development. BMP-7 works synergistically with BMP-2 to play a key role in the differentiation of mesenchymal cells into bone and cartilage tissues by actively recruiting stem cells from the surrounding tissue, thereby initiating the bone formation cascade. This antibody has been validated in immunohistochemistry (right) and Western blotting applications in both human and mouse samples.

Description	Qty/Pk	Catalogue Number
Anti-BMP-7, clone 2A10	100 μg	MAB4350

Anti-SRF (Serum Response Factor)

Serum response factor (SRF) is a nuclear transcription factor that is essential for embryonic development of the mesoderm, as well as for the maintenance of adult muscle cells and neurons. As this protein is involved in the downstream regulation of many immediate-early genes, it participates in a variety of cell functions, including cell cycle, apoptosis, cell growth, and cell differentiation. This antibody has been validated in immunocytochemistry (right) and Western blotting applications in both human and mouse samples.

Description	Qty/Pk	Catalogue Number
Anti-SRF (Serum Response Factor), clone 1E1	100 μg	MAB4369

Stem Cell Characterization Tools

Human Mesenchymal Stem Cell (MSC) Characterization Kit

The human MSC characterization kit contains a panel of positive and negative selection markers for the comprehensive characterization of mesenchymal stem cell populations in human samples. Positive markers include antibodies to cell-surface molecules including CD44, CD90, STRO-1, and CD146. In addition, two specific hematopoietic cell surface markers are provided as negative markers: CD14 (present on leukocytes) and CD19 (present on B-lymphocytes). The kit provides a convenient tool for fully and definitively characterizing human mesenchymal stem cells.

Description	Qty/Pk	Catalogue Number
Human Mesenchymal Stem Cell Characterization Kit	1 kit	SCR067

ELISPOT Reagents

ELISPOT Antibody Pairs

ELISPOT enzyme-linked immunospot assays are an effective method for the qualitative and quantitative analysis of immune response patterns, and can be used to identify antigenic peptides for the creation of vaccine strategies. This assay has the sensitivity to identify antigen-specific T or B cells on a single cell level with results that closely mirror in vivo conditions, thereby eliminating the need for long-term in vitro cell cultures or the addition of exogenous cytokines. Each Millipore ELISPOT antibody pair contains sufficient reagents for five 96-well plates: 500 μg capture antibody, 100 μg biotinylated detection antibody, and 55 μL streptavidin-conjugated alkaline phosphatase. The ELISPOT antibody pairs have been tested on both MultiScreen_HTS-IP filter plates and 8-well strip formats.

Description	Catalogue Number
IL-2 ELISPOT Antibody Pair, human	ELI-002-H
IL-2 ELISPOT Antibody Pair, mouse	ELI-002-M
IL-4 ELISPOT Antibody Pair, human	ELI-004-H
IL-4 ELISPOT Antibody Pair, mouse	ELI-004-M
IL-5 ELISPOT Antibody Pair, human	ELI-006-H
IL-5 ELISPOT Antibody Pair, mouse	ELI-006-M
IL-6 ELISPOT Antibody Pair, human	ELI-008-H
IL-6 ELISPOT Antibody Pair, mouse	ELI-008-M
IL-10 ELISPOT Antibody Pair, human	ELI-010-H
GM-CSF ELISPOT Antibody Pair, human	ELI-012-H
IL-12p40 ELISPOT Antibody Pair, mouse	ELI-014-M
IFNγ ELISPOT Antibody Pair, human	ELI-016-H
IFNγ ELISPOT Antibody Pair, mouse	ELI-016-M
TNFα ELISPOT Antibody Pair, human	ELI-018-H
TNFα ELISPOT Antibody Pair, mouse	ELI-018-M

Cultureware

Millicell Tissue Culture Treated Plates

Millipore’s Millicell tissue culture plates are high quality, injection molded polystyrene plates that have numerous advantages over plastic plates, especially for epithelial and endothelial cell culture. Millicell tissue culture plates are provided sterile and ready-to-use for direct and co-culture applications, and are designed to work with our Millicell hanging and standing inserts.

Description	Plate Size	Qty/Pk	Catalogue Number
Millicell Cell Culture Receiver Plate, tissue culture treated, sterile	6-Well	50/pk	PIMWS0650
Millicell Cell Culture Receiver Plate, tissue culture treated, sterile	12-well	50/pk	PIMWS1250
Millicell Cell Culture Receiver Plate, tissue culture treated, sterile	24-well	50/pk	PIMWS2450

Millicoat ECM-Coated Cultureware

Millicoat plates are standard 6- and 24-well plates that have been precoated with one of several of the most common ECMs used in cell culture studies. Compared to traditional self-coating methods that require long ECM drying times and careful techniques, Millicoat plates provide consistent gel thickness and uniform coating in a convenient, ready-to-use format.

Description	Plate Size	Qty/Pk	Catalogue Number
Millicoat plate coated with collagen 1	6-well	5	PICL06P05
Millicoat plate coated with collagen 1	24-well	5	PICL24P05
Millicoat plate coated with poly-D-lysine	6-well	5	PIDL06P05
Millicoat plate coated with poly-D-lysine	24-well	5	PIDL24P05
Millicoat plate coated with fibronectin	6-well	5	PIFB06P05
Millicoat plate coated with fibronectin	24-well	5	PIFB24P05