QCM Chemotaxis Cell Migration Assays
Chemotaxis assays are ideal for assessing the effects of pharmacological compounds on the motility of tumor cells, and for analyzing the migratory capacity of multiple cell lines in parallel. The format options enable large-scale screening and quantitative comparison of multiple samples in a user friendly, sensitive, HTS system.
QCM Chemotaxis Cell Migration Assays are built on the platform of a multiwell plate that includes a 24- or 96-well migration chamber and a feeder tray. Each chamber contains a microporous polycarbonate membrane of a specified pore size. Both sides of the membrane are uncoated. Cells of interest are pipetted onto the top of the insert. Migratory cells move through the pores of the membrane and cling to the bottom of the polycarbonate membrane, in response to a chemoattractant loaded into the bottom feeder tray.
Fluorescent Assays
Migrated cells are detached, lysed, and labeled with a fluorescent dye that exhibits strong fluorescence when bound to cellular nucleic acids. Sample fluorescence is measured with a fluorescence microplate reader. The excitation maximum is 480 nm; the emission maximum is 520 nm.
Colorimetric Assays
Migrated cells on the bottom of the plate are stained and either viewed with a light microscope or extracted and detected on a standard microplate reader (560 nm).
Pore Size Options
8 µm – suitable for fibroblasts, endothelial cells
5 µm – suitable for monocytes, macrophages
3 µm – suitable for lymphocytes
Human Fibrosarcoma HT-1080 Chemotaxis Assay. HT-1080 Chemotaxis toward 10% FBS was tested in the presence or absence of 10 µm Cytochalasin D. 40,000 cells were used in each assay. Fluorescence measurements were taken at 2, 4, or 24 hours according to assay instructions. |
Kit Components
Colorimetric Assay- Boyden Chamber Plate Assembly with porous membrane inserts
- Cell Stain Solution
- Extraction Buffer
- Boyden Chamber Plate Assembly with porous membrane inserts
- Cell Detachment Buffer
- Lysis Buffer
- CyQuant® GR Dye
Product News & Releases
- BioTime Announces Stem Cell Co-marketing Agreement with Millipore
- Millipore Supports Executive Order on Stem Cell Research
- Faster Flow Rates for Sterile Filtration - New Millex® syringe filters with Millipore Express PLUS (PES) membrane
- ReNcell® VM Neural Stem Cell Line Models Parkinson’s Disease - New human disease model shows function of PINK1 gene in neural degeneration
Featured Products

- Fast-Trap Lentivirus Purification and Concentration Kit
- Fast-Trap Adenovirus Purification and Concentration Kit
- Guava Flow Cytometry Systems
- ECM Cell Culture Optimization Arrays
- ESGRO Complete
- GCTM-5 Antibody, clone GCTM-5
- Guava Viacount Assay
- MilliTrace Constitutive GFP Reporter
- MultiScreenHTS + and MultiScreen Vacuum Manifolds
- ENStem-A hES-derived Human Neural Progenitors
- Millicell Cell Culture Insert




