QCM Chemotaxis Cell Migration Assays

Quantitative fluorescent method increases sensitivity 10-fold over similar assays on the market
Does not require cell pre-labeling, cell scraping, or cell counting
Results in 4–24 hours
96-well plate is ideal for high throughput screening

Chemotaxis assays are ideal for assessing the effects of pharmacological compounds on the motility of tumor cells, and for analyzing the migratory capacity of multiple cell lines in parallel. The format options enable large-scale screening and quantitative comparison of multiple samples in a user friendly, sensitive, HTS system.

QCM Chemotaxis Cell Migration Assays are built on the platform of a multiwell plate that includes a 24- or 96-well migration chamber and a feeder tray. Each chamber contains a microporous polycarbonate membrane of a specified pore size. Both sides of the membrane are uncoated. Cells of interest are pipetted onto the top of the insert. Migratory cells move through the pores of the membrane and cling to the bottom of the polycarbonate membrane, in response to a chemoattractant loaded into the bottom feeder tray.

Fluorescent Assays

Migrated cells are detached, lysed, and labeled with a fluorescent dye that exhibits strong fluorescence when bound to cellular nucleic acids. Sample fluorescence is measured with a fluorescence microplate reader. The excitation maximum is 480 nm; the emission maximum is 520 nm.

Colorimetric Assays

Migrated cells on the bottom of the plate are stained and either viewed with a light microscope or extracted and detected on a standard microplate reader (560 nm).

Pore Size Options

8 µm – suitable for fibroblasts, endothelial cells
5 µm – suitable for monocytes, macrophages
3 µm – suitable for lymphocytes

Human Fibrosarcoma HT-1080 Chemotaxis Assay.

HT-1080 Chemotaxis toward 10% FBS was tested in the presence or absence of 10 µm Cytochalasin D. 40,000 cells were used in each assay. Fluorescence measurements were taken at 2, 4, or 24 hours according to assay instructions.

Kit Components

Colorimetric Assay

Boyden Chamber Plate Assembly with porous membrane inserts
Cell Stain Solution
Extraction Buffer

Fluorimetric Assay

Boyden Chamber Plate Assembly with porous membrane inserts
Cell Detachment Buffer
Lysis Buffer
CyQuant® GR Dye

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