QCM Haptotaxis Cell Migration Assays

Millipore’s Haptotaxis Cell Migration Assays measure cell movement toward an immobilized ECM protein gradient. The Boyden chambers have been outwardly pre-coated with an appropriate ECM or BSA (negative control) to allow optimal cell migration. An 8 µm pore size supports the optimal haptotactic migration of epithelial and fibroblast cells. These assays allow quantitative analysis using a standard microplate reader.

Microporous chambers pre-coated with ECM proteins eliminate the need for overnight coating
Quantitative assay for easy comparison of numeric values from multiple experiments
Built-in migration and adhesion controls for each sample
Optimized pre-coated plates reduce inter-assay variation

Kit Components

Colorimetric

24-well Plate containing 12 ECM-coated Boyden Chambers
24-well Control Plate containing 12 BSA-coated Boyden Chambers
24-well Stain Extraction Plate
96-well Quantitation Plate
Cell Stain Solution
Extraction Buffer
Swabs
Forceps

Fluorimetric

ECM-Coated Boyden Chamber Plate Assembly
BSA-Coated Boyden Chamber Plate Assembly
Transfer Plate(s)
Lysis Buffer
CyQuant® GR Dye

Cell Migration Colorimetric Assay Principle

Steps

Harvest subject cells, pellet, and resuspend to 0.75–2.5 x 106 cells per mL.
Place 125–500 µL of cells per well in ECM-coated test chambers, BSA control chambers, and ECM-coated control wells.
Incubate for 2–24 hours in a CO2 incubator.
Stain ECM-coated control wells and visualize with a microscope to confirm attachment morphology. Remove non-migrating cells from coated chambers with a swab.
Stain migration chambers, and rinse away excess stain.
Solubilize stained migratory cells with extraction buffer.
Transfer 50–150 µL of extraction buffer from wells to microplate, and read OD 540–570 nm.
Plot OD, correlating with migration.

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